Abnova™ Fibrinogen (Human) ELISA Kit

Description

Includes

• Human FBG Microplate: A 96-well polystyrene microplate coated with a polyclonal antibody against human FBG
• Sealing Tapes
• Human FBG Standard: Human FBG in a buffered protein base (lyophilized)
• Biotinylated Human FBG Antibody (50x)
• MIX Diluent Concentrate (10x)
• Wash Buffer Concentrate (20x)
• Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate
• Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine
• Stop Solution: A 0.5N hydrochloric acid

This assay employs a quantitative sandwich enzyme immunoassay technique that measures human FBG in less than 4 hours

Specifications

• Calibration Range: 1.25 to 80ng/mL
• Conjugate: Biotin
• Cross Reactivity: Human
• Description: Fibrinogen (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human fibrinogen in urine and cell culture supernatant.
• Detection Limit: 1ng/mL
• Detection Method: Colorimetric
• Includes: Antibody coated microwells (96 wells), Human FBG Standard (400ng), Biotinylated FBG Antibody (100x): 1 vial, MIX Diluent Concentrate (10x): 30mL, Wash Buffer Concentrate (20x): 30mL x 2, Streptavidin-Peroxidase Conjugate (SP Conjugate): 80, Chromogen Substrate (8mL), Stop Solution (12mL)
• Quantity: 1 Kit
• Regulatory Status: For research use only (RUO)
• Sample Type: Cell Culture Samples, CSF, Milk, Saliva, Urine
• Sample Volume: 50 μL
• Packaging: 96-well
• Content And Storage: Store the kit at -20°C. The reconstituted standard, SP Conjugate and biotinylated antibody should be stored at -20°C. Others stored at 4°C. Aliquot to avoid repeated freezing and thawing.
• For Use With (Application): Quantification
• Product Type: ELISA Kit
• No. of Assays: 96