Learn More
Description
Protein conjugates prepared with Fluorescence Dye 350-I are bright with fluorescence unaffected by pH in the physiological range (pH 4 to 10). Ideal for labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1 to 20g/mL is sufficient. The optimal working dilution should be determined by the end user.
- Spectral Equivalent: Alexa Fluor 350, AMCA, DyLight 350
- Molar Extinction Co-efficient: >80,000 M-1cm-1
- Excitation (Max): 345nm
- Emission (Max): 442nm
- Solubility: Water
- Storage Buffer: Water
Application: Conjugation
Recommended Storage: Store in desiccated environment at -20°C and avoid from light.
Specifications
Specifications
| Description | Fluorescence Dye 350-I with Streptavidin conjugate. Protein conjugates prepared with Fluorescence Dye 350-I are bright with fluorescence unaffected by pH in the physiological range (pH 4-10). |
| For Use With (Application) | Conjugation |
| Quantity | 200 μg |
| Product Type | Fluorescent Dye 350-I Streptavidin |
| Equivalence | Alexa Fluor 350, AMCA, DyLight 350 |
| Storage Buffer | Water |
| Content And Storage | Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C. |
| Label or Dye | Fluorescent Dye 350-I Streptavidin |
| Emission | 442 |
| Excitation | 345 |
For research use only (RUO).
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.