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Abnova™ Fluorescent Dye 405-I Streptavidin
Description
Protein conjugates prepared with Fluorescence Dye 405-I are bright with fluorescence unaffected by pH in the physiological range (pH 4-10). Fluorescence Dye 405-I labeled conjugates are excellent violet laser reagents for flow cytometry research. Ideal for labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1 to 20g/mL is sufficient. The optimal working dilution should be determined by the end user.
- Spectral Equivalent: Alexa Fluor 405, DyLight 405
- Molar Extinction Co-efficient: ≥20,000 M-1cm-1
- Excitation (Max): 401nm
- Emission (Max): 420nm
- Solubility: water
- Storage Buffer: Water
Application: Conjugation, Flow Cytometry
Recommended Storage: Store in desiccated environment at -20°C and avoid from light.
Specifications
Specifications
Description | Fluorescence Dye 405-I with Streptavidin conjugate. Protein conjugates prepared with Fluorescence Dye 405-I are bright with fluorescence unaffected by pH in the physiological range (pH 4-10). Fluorescence Dye 405-I labeled conjugates are excellent violet laser reagents for flow cytometry research. |
For Use With (Application) | Conjugation; Flow Cytometry |
Quantity | 200 μg |
Product Type | Fluorescent Dye 405-I Streptavidin |
Equivalence | Alexa Fluor 405, DyLight 405 |
Storage Buffer | Water |
Content And Storage | Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C. |
Label or Dye | Fluorescent Dye 405-I Streptavidin |
Emission | 420 |
Excitation | 401 |
For research use only (RUO).
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