Abnova™ Fluorescent Dye 405-I Streptavidin

Description

Fluorescence Dye 405-I with Streptavidin conjugate

Protein conjugates prepared with Fluorescence Dye 405-I are bright with fluorescence unaffected by pH in the physiological range (pH 4-10). Fluorescence Dye 405-I labeled conjugates are excellent violet laser reagents for flow cytometry research. Ideal for labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1 to 20g/mL is sufficient. The optimal working dilution should be determined by the end user.

• Spectral Equivalent: Alexa Fluor 405, DyLight 405
• Molar Extinction Co-efficient: ≥20,000 M^-1cm^-1
• Excitation (Max): 401nm
• Emission (Max): 420nm
• Solubility: water
• Storage Buffer: Water

Application: Conjugation, Flow Cytometry

Recommended Storage: Store in desiccated environment at -20°C and avoid from light.

Specifications

• Description: Fluorescence Dye 405-I with Streptavidin conjugate. Protein conjugates prepared with Fluorescence Dye 405-I are bright with fluorescence unaffected by pH in the physiological range (pH 4-10). Fluorescence Dye 405-I labeled conjugates are excellent violet laser reagents for flow cytometry research.
• For Use With (Application): Conjugation; Flow Cytometry
• Quantity: 200 μg
• Product Type: Fluorescent Dye 405-I Streptavidin
• Equivalence: Alexa Fluor 405, DyLight 405
• Storage Buffer: Water
• Content And Storage: Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.
• Label or Dye: Fluorescent Dye 405-I Streptavidin
• Emission: 420
• Excitation: 401

For research use only (RUO).