Abnova™ Fluorescent Dye 488-I Streptavidin

Description

Fluorescence Dye 488-I with Streptavidin conjugate

Fluorescence Dye 488-I conjugates are significantly brighter than fluorescein conjugates and much more photostable. Additionally, the fluorescence of Fluorescence Dye 488-I is not affected by pH between 4-10. Compared to Alexa Fluor 488, Fluorescence Dye 488-I of single isomer has much higher purity, bringing better performance consistency from batch to batch. Ideal for labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1 to 20g/mL is sufficient. The optimal working dilution should be determined by the end user.

• Spectral Equivalent: Alexa Fluor 488, Cy2, DyLight 488, FITC
• Molar Extinction Co-efficient: ≥70,000 M^-1cm^-1
• Excitation (Max): 491nm
• Emission (Max): 514nm
• Solubility: Water
• Storage Buffer: Water

Application: Conjugation

Recommended Storage: Store in desiccated environment at -20°C and avoid from light.

Specifications

• Description: Fluorescence Dye 488-I with Streptavidin conjugate. Fluorescence Dye 488-I conjugates are significantly brighter than fluorescein conjugates and much more photostable. Additionally, the fluorescence of Fluorescence Dye 488-I is not affected by pH between 4-10. Compared to Alexa Fluor 488, “Fluorescence Dye 488-I of single isomer has much hihger purity, bringing better performance consistancy from batch to batch.
• For Use With (Application): Conjugation
• Quantity: 200 μg
• Product Type: Fluorescent Dye 488-I Streptavidin
• Equivalence: Alexa Fluor 488, Cy2, DyLight 488, FITC
• Storage Buffer: Water
• Content And Storage: Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.
• Label or Dye: Fluorescent Dye 488-I Streptavidin
• Emission: 514
• Excitation: 491

For research use only (RUO).