Abnova™ Fluorescent Dye 540-M Streptavidin

Fluorescence Dye 540-M with Streptavidin conjugate

Supplier:  Abnova™ U0287

Catalog No. 89-139-573

Description

Fluorescent Dye 540-M may have less spillover effect than Pacific Orange due to its slightly sharper emission. All the Fluorescent Dye M series products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. Besides, in comparison with phycobiliprotein tandems, Fluorescent Dye M series possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications. Ideal for labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1 to 20g/mL is sufficient. The optimal working dilution should be determined by the end user.

• Spectral Equivalent: Krome Orange, Pacific Orange
• Recommended Excitation: Violet (405nm)
• Recommended Filter Sets: Pacific Orange Filter
• Molar Extinction Co-efficient: ≥18,000 M^-1cm^-1
• Excitation (Max): 405nm
• Emission (Max): 537nm
• Solubility: Water
• Storage Buffer: Water

Application: Conjugation, Flow Cytometry

Recommended Storage: Store in desiccated environment at -20°C and avoid from light.

Specifications

• Description: Fluorescence Dye 540-M with Streptavidin conjugate. Fluorescent Dye 540-M may have less spillover effect than Pacific Orange due to its slightly sharper emission. All the Fluorescent Dye M series products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. Besides, in comparison with phycobiliprotein tandems, Fluorescent Dye M series possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications.
• Quantity: 100 μg
• Filter Type: Pacific Orange Filter
• Storage Buffer: Water
• Label or Dye: Fluorescent Dye 540-M Streptavidin
• Excitation: 405

• For Use With (Application): Conjugation; Flow Cytometry
• Product Type: Fluorescent Dye 540-M Streptavidin
• Equivalence: Krome Orange, Pacific Orange
• Content And Storage: Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.
• Emission: 537

For research use only (RUO).