Abnova™ Fluorescent Dye 555-I Streptavidin

Description

Fluorescence Dye 555-I with Streptavidin conjugate

Compared with Cy3 conjugates, those of Fluorescence Dye 555-I are slightly red-shifted, resulting in an optimal match to filters designed for Cy3 dyes in accompany with improved photostability. Besides, their fluorescence is pH-independent from pH 3 to 11. Ideal for labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1 to 20g/mL is sufficient. The optimal working dilution should be determined by the end user.

• Spectral Equivalent: Alexa Fluor 555, Cy3, DyLight 550, TRITC
• Molar Extinction Co-efficient: ≥1,00,000 M^-1cm^-1
• Excitation (Max): 555nm
• Emission (Max): 565nm
• Solubility: Water
• Storage Buffer: Water

Application: Conjugation

Recommended Storage: Store in desiccated environment at -20°C and avoid from light.

Specifications

• Description: Fluorescence Dye 555-I with Streptavidin conjugate. Compared with Cy3 conjugates, those of Fluorescence Dye 555-I are slightly red-shifted, resulting in an optimal match to filters designed for Cy3 dyes in accompany with improved photostability. Besides, their fluorescence is pH-independent from pH 3 to 11.
• For Use With (Application): Conjugation
• Quantity: 200 μg
• Product Type: Fluorescent Dye 555-I Streptavidin
• Equivalence: Alexa Fluor 555, Cy3, DyLight 550, TRITC
• Storage Buffer: Water
• Content And Storage: Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.
• Label or Dye: Fluorescent Dye 555-I Streptavidin
• Emission: 565
• Excitation: 555

For research use only (RUO).