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Description
Compared with Cy3 conjugates, those of Fluorescence Dye 555-I are slightly red-shifted, resulting in an optimal match to filters designed for Cy3 dyes in accompany with improved photostability. Besides, their fluorescence is pH-independent from pH 3 to 11. Ideal for labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1 to 20g/mL is sufficient. The optimal working dilution should be determined by the end user.
- Spectral Equivalent: Alexa Fluor 555, Cy3, DyLight 550, TRITC
- Molar Extinction Co-efficient: ≥1,00,000 M-1cm-1
- Excitation (Max): 555nm
- Emission (Max): 565nm
- Solubility: Water
- Storage Buffer: Water
Application: Conjugation
Recommended Storage: Store in desiccated environment at -20°C and avoid from light.
Specifications
Specifications
| Description | Fluorescence Dye 555-I with Streptavidin conjugate. Compared with Cy3 conjugates, those of Fluorescence Dye 555-I are slightly red-shifted, resulting in an optimal match to filters designed for Cy3 dyes in accompany with improved photostability. Besides, their fluorescence is pH-independent from pH 3 to 11. |
| For Use With (Application) | Conjugation |
| Quantity | 200 μg |
| Product Type | Fluorescent Dye 555-I Streptavidin |
| Equivalence | Alexa Fluor 555, Cy3, DyLight 550, TRITC |
| Storage Buffer | Water |
| Content And Storage | Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C. |
| Label or Dye | Fluorescent Dye 555-I Streptavidin |
| Emission | 565 |
| Excitation | 555 |
For research use only (RUO).
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