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Description
Biomolecules conjugated to Fluorescence Dye 594-I exhibit little spectral overlap with green-fluorescent conjugates, and can be efficiently excited by 568nm line of Ar-Kr laser and by the 594nm line of orange He-Ne laser. Ideal for labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1 to 20g/mL is sufficient. The optimal working dilution should be determined by the end user.
- Spectral Equivalent: Alexa Fluor 594, DyLight 594, Texas Red
- Molar Extinction Co-efficient: ≥80,000 M-1cm-1
- Excitation (Max): 592nm
- Emission (Max): 614nm
- Solubility: water
- Storage Buffer: water
Application: Conjugation
Recommended Storage: Store in desiccated environment at -20°C and avoid from light.
Specifications
Specifications
| Description | Fluorescence Dye 594-I with Streptavidin conjugate. Biomolecules conjugated to Fluorescence Dye 594-I exhibit little spectral overlap with green-fluorescent conjugates, and can be efficiently excited by 568nm line of Ar-Kr laser and by the 594nm line of orange He-Ne laser. |
| For Use With (Application) | Conjugation |
| Quantity | 200 μg |
| Product Type | Fluorescent Dye 594-I Streptavidin |
| Equivalence | Alexa Fluor 594, DyLight 594, Texas Red |
| Storage Buffer | Water |
| Content And Storage | Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C. |
| Label or Dye | Fluorescent Dye 594-I Streptavidin |
| Emission | 614 |
| Excitation | 592 |
For research use only (RUO).
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