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Description
All the Fluorescent Dye M series products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. Besides, in comparison with phycobiliprotein tandems, Fluorescent Dye M series possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications. Ideal for labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1 to 20g/mL is sufficient. The optimal working dilution should be determined by the end user.
- Molar Extinction Co-efficient: ≥50,000 M-1cm-1
- Recommended Excitation: Green (532nm)
- Excitation (Max): 523nm
- Emission (Max): 617nm
- Solubility: Water
- Storage Buffer: Water
Application: Conjugation, Flow Cytometry
Recommended Storage: Store in desiccated environment at -20°C and avoid from light.
Specifications
Specifications
| Description | Fluorescence Dye 620-M with Streptavidin conjugate. All the Fluorescent Dye M series products are designed to be maximally excited by one of the major light sources equipped in flow cytometers. Besides, in comparison with phycobiliprotein tandems, Fluorescent Dye M series possesses better photostability, higher conjugation yields, and little pH sensitivity making it an ideal choice in fluorescence imaging applications. |
| For Use With (Application) | Conjugation; Flow Cytometry |
| Quantity | 100 μg |
| Product Type | Fluorescent Dye 620-M Streptavidin |
| Storage Buffer | Water |
| Content And Storage | Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C. |
| Label or Dye | Fluorescent Dye 620-M Streptavidin |
| Emission | 617 |
| Excitation | 523 |
For research use only (RUO).
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