Abnova™ Fluorescent Dye 633-I Streptavidin

Description

Fluorescence Dye 633-I with Streptavidin conjugate

Fluorescence emission of Fluorescence Dye 633-I is well separated from that of other commonly used red fluorophores, such as TAMRA, Texas Red, Alexa Fluor 594, Fluorescent Dye 594-I and R-phycoerythrin, making it ideal for multi-color labeling. In comparison with Alexa Fluor 633, the extinction coefficient of Fluorescence Dye 633-I is much higher. Ideal for labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1 to 20g/mL is sufficient. The optimal working dilution should be determined by the end user.

• Spectral Equivalent: Alexa Fluor 633, DyLight 633
• Molar Extinction Co-efficient: ≥2,40,000 M^-1cm^-1
• Excitation (Max): 638nm
• Emission (Max): 655nm
• Solubility: Water
• Storage Buffer: Water

Application: Conjugation, Flow Cytometry

Recommended Storage: Store in desiccated environment at -20°C and avoid from light.

Specifications

• Description: Fluorescence Dye 633-I with Streptavidin conjugate. Fluorescence emission of Fluorescence Dye 633-I is well separated from that of other commonly used red fluorophores, such as TAMRA, Texas Red, Alexa Fluor 594, Fluorescent Dye 594-I and R-phycoerythrin, making it ideal for multi-color labeling. In comparison with Alexa Fluor 633, the extinction coefficient of Fluorescence Dye 633-I is much higher (250,000 cm^-1M^-1).
• For Use With (Application): Conjugation; Flow Cytometry
• Quantity: 200 μg
• Product Type: Fluorescent Dye 633-I Streptavidin
• Equivalence: Alexa Fluor 633, DyLight 633
• Storage Buffer: Water
• Content And Storage: Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.
• Label or Dye: Fluorescent Dye 633-I Streptavidin
• Emission: 655
• Excitation: 638

For research use only (RUO).