Abnova™ Fluorescent Dye 647-I Streptavidin

Catalog No.	Quantity
89-139-583	200 μg

Catalog No. 89-139-583 Supplier Abnova™ Supplier No. U0297

Description

Fluorescence Dye 647-I with Streptavidin conjugate

The slight red shift in absorption spectrum makes Fluorescence Dye 647-I an optimal match to filters designed Cy5 dyes. Besides, their fluorescence is pH-independent from pH 3 to 11. Ideal for labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1 to 20g/mL is sufficient. The optimal working dilution should be determined by the end user.

• Spectral Equivalent: Alexa Fluor 647, Cy5, DyLight 650
• Molar Extinction Co-efficient: ≥2,40,000 M^-1cm^-1
• Excitation (Max): 649nm
• Emission (Max): 664nm
• Solubility: Water
• Storage Buffer: Water

Application: Conjugation, Flow Cytometry

Recommended Storage: Store in desiccated environment at -20°C and avoid from light.

Specifications

• Description: Fluorescence Dye 647-I with Streptavidin conjugate. The slight red shift in absorption spectrum makes Fluorescence Dye 647-I an optimal match to filters designed Cy5 dyes.Besides, their fluorescence is pH-independent from pH 3 to 11.
• For Use With (Application): Conjugation; Flow Cytometry
• Quantity: 200 μg
• Product Type: Fluorescent Dye 647-I Streptavidin
• Equivalence: Alexa Fluor 647, Cy5, DyLight 650
• Storage Buffer: Water
• Content And Storage: Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.
• Label or Dye: Fluorescent Dye 647-I Streptavidin
• Emission: 664
• Excitation: 649

For research use only (RUO).