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Abnova™ Fluorescent Dye 680-I Streptavidin

Fluorescence Dye 680-I with Streptavidin conjugate

Manufacturer:  Abnova™ U0299

Catalog No. 89-139-585


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Description

Description

Fluorescence emission of Fluorescence Dye 680-I is well separated from that of other commonly used red fluorophores, such as TAMRA, Texas Red, Alexa Fluor 594, Fluorescent Dye 594-I, Fluorescent Dye 633-I, Fluorescent Dye 647-I and R-phycoerythrin, making it ideal for multi-color labeling. Besides, their fluorescence is pH-independent from pH 3 to 11. Ideal for labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1 to 20g/mL is sufficient. The optimal working dilution should be determined by the end user.

  • Spectral Equivalent: Alexa Fluor 680, Cy5.5, IRDye 700
  • Molar Extinction Co-efficient: ≥2,00,000 M-1cm-1
  • Excitation (Max): 676nm
  • Emission (Max): 695nmnm
  • Solubility: Water
  • Storage Buffer: Water

Application: Conjugation, Flow Cytometry

Recommended Storage: Store in desiccated environment at -20°C and avoid from light.

Specifications

Specifications

Fluorescence Dye 680-I with Streptavidin conjugate. Fluorescence emission of Fluorescence Dye 680-I is well separated from that of other commonly used red fluorophores, such as TAMRA, Texas Red, Alexa Fluor 594, Fluorescent Dye 594-I, Fluorescent Dye 633-I, Fluorescent Dye 647-I and R-phycoerythrin, making it ideal for multi-color labeling. Besides, their fluorescence is pH-independent from pH 3 to 11.
Fluorescent Dye 680-I Streptavidin
Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.
676nm
Alexa Fluor 680, Cy5.5, IRDye 700
Conjugation; Flow Cytometry
Fluorescent Dye 680-I Streptavidin
200 ug
695nm
Water
SDS
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