Abnova™ Fluorescent Dye 750-I Streptavidin

Catalog No.	Quantity
89-139-590	200 μg

Catalog No. 89-139-590 Supplier Abnova™ Supplier No. U0304

Description

Fluorescence Dye 750-I with Streptavidin conjugate

Compared with Alexa Fluor 750, Fluorescent Dye 750-I is much brighter with absorption at 633nm. Fluorescence emission of Fluorescence Dye 750-I is well separated from that of other commonly used red fluorophores, such as TAMRA, Fluorescent Dye 647-I and R-phycoerythrin, making it ideal for multi-color labeling. Ideal for labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1 to 20g/mL is sufficient. The optimal working dilution should be determined by the end user.

• Spectral Equivalent: Alexa Fluor 750, Cy7, DyLight 755
• Molar Extinction Co-efficient: ≥2,40,000 M^-1cm^-1
• Excitation (Max): 749nm
• Emission (Max): 775nm
• Solubility: Water
• Storage Buffer: Water

Application: Conjugation, Flow Cytometry

Recommended Storage: Store in desiccated environment at -20°C and avoid from light.

Specifications

• Description: Fluorescence Dye 750-I with Streptavidin conjugate. Compared with Alexa Fluor 750, Fluorescent Dye 750-I is much brighter with absorption at 633nm. Fluorescence emission of Fluorescence Dye 750-I is well separated from that of other commonly used red fluorophores, such as TAMRA, Fluorescent Dye 647-I and R-phycoerythrin, making it ideal for multi-color labeling.
• For Use With (Application): Conjugation; Flow Cytometry
• Quantity: 200 μg
• Product Type: Fluorescent Dye 750-I Streptavidin
• Equivalence: Alexa Fluor 750, Cy7, DyLight 755
• Storage Buffer: Water
• Content And Storage: Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.
• Label or Dye: Fluorescent Dye 750-I Streptavidin
• Emission: 775
• Excitation: 749

For research use only (RUO).