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Description
Fluorescent Dye Eu is optimized for TR-FRET applications with large Stokes shifts and extremely long emission half-lives when compared to traditional fluorophores such as Alexa Fluor™ or Cyanine dyes. Ideal for labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1 to 20g/mL is sufficient. The optimal working dilution should be determined by the end user.
- Typical Acceptor for TR-FRET: Alexa 647, APC, Cy5, DyLight 650, Fluorescence Dye 647-I, TF5
- Molar Extinction Co-efficient: ≥22,000 M-1cm-1
- Excitation (Max): 346nm
- Emission (Max): 617nm
- Solubility: Water
- Storage Buffer: Water
Application: Conjugation, Time Resolved-Fluorescence Resonance Energy Transfer
Recommended Storage: Store in desiccated environment at -20°C and avoid from light.
Specifications
Specifications
| Description | Fluorescent Dye Eu with Streptavidin conjugate. Fluorescent Dye Eu is optimized for TR-FRET applications with large Stokes shifts and extremely long emission half-lives when compared to traditional fluorophores such as Alexa Fluor™ or Cyanine dyes. |
| For Use With (Application) | Conjugation; Time Resolved-Fluorescence Resonance Energy Transfer |
| Quantity | 100 μg |
| Product Type | Fluorescent Dye Eu-Streptavidin |
| Equivalence | Alexa 647, APC, Cy5, DyLight 650, Fluorescence Dye 647-I, TF5 |
| Storage Buffer | Water |
| Content And Storage | Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C. |
| Label or Dye | Fluorescent Dye Eu-Streptavidin |
| Emission | 617 |
| Excitation | 346 |
For research use only (RUO).
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