Abnova™ Fluorescent Dye Eu-Streptavidin

Description

Fluorescent Dye Eu with Streptavidin conjugate

Fluorescent Dye Eu is optimized for TR-FRET applications with large Stokes shifts and extremely long emission half-lives when compared to traditional fluorophores such as Alexa Fluor™ or Cyanine dyes. Ideal for labeling via Streptavidin for fluorescence imaging and other fluorescence-based biochemical analysis. For most fluorescent streptavidin applications, a concentration of 1 to 20g/mL is sufficient. The optimal working dilution should be determined by the end user.

• Typical Acceptor for TR-FRET: Alexa 647, APC, Cy5, DyLight 650, Fluorescence Dye 647-I, TF5
• Molar Extinction Co-efficient: ≥22,000 M^-1cm^-1
• Excitation (Max): 346nm
• Emission (Max): 617nm
• Solubility: Water
• Storage Buffer: Water

Application: Conjugation, Time Resolved-Fluorescence Resonance Energy Transfer

Recommended Storage: Store in desiccated environment at -20°C and avoid from light.

Specifications

• Description: Fluorescent Dye Eu with Streptavidin conjugate. Fluorescent Dye Eu is optimized for TR-FRET applications with large Stokes shifts and extremely long emission half-lives when compared to traditional fluorophores such as Alexa Fluor™ or Cyanine dyes.
• For Use With (Application): Conjugation; Time Resolved-Fluorescence Resonance Energy Transfer
• Quantity: 100 μg
• Product Type: Fluorescent Dye Eu-Streptavidin
• Equivalence: Alexa 647, APC, Cy5, DyLight 650, Fluorescence Dye 647-I, TF5
• Storage Buffer: Water
• Content And Storage: Store the product in desiccated environment at -20°C and avoid from light. Add ddH2O with 0.1% BSA into the vial to have 1 mg/mL stock solution. This reconstituted solution remains stable for 2 months when stored in the presence of 2 mM sodium azide and avoid from light at 4°C. For longer storage, divid the reconstituted solution into single-used aliquots, or add equal volume of glycerol without aliquoting, and avoid from light at -20°C.
• Label or Dye: Fluorescent Dye Eu-Streptavidin
• Emission: 617
• Excitation: 346

For research use only (RUO).