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Description
Includes
- Micro titer strips Divisible microplate consisting of 12 modules of 8 wells each, coated with highly purified GBM. Ready to use. 12
- Anti-GBM Calibrators (A-E) in a serum/buffer matrix (PBS, BSA, NaN3 <0.1% (w/w)) containing: 0; 20; 40; 80; 200U/mL Ready to use. 5 vials, 1.5mL each
- Anti-GBM Controls in a serum/buffer matrix (PBS, BSA, NaN3 <0.1% (w/w)) positive (1) and negative (2), for the respective concentrations see the enclosed package insert. Ready to use. 2 vials, 1.5mL each
- Sample buffer (Tris, NaN3 <0.1% (w/w)), yellow, concentrate (5x). 1 vial, 20mL
- Enzyme Conjugate (PBS, Proclin 300 <0.5% (v/v)), (light red) containing polyclonal rabbit anti-human IgG; labelled with horseradish peroxidase. Ready to use. 1 vial, 15mL
- TMB substrate solution TMB substrate solution. Ready to use. 1 vial, 15mL
- Wash solution (PBS, NaN3 <0.1% (w/w)), concentrate (50x). 1 vial, 15mL
Specifications
Specifications
| Conjugate | HRP |
| Cross Reactivity | Human |
| Description | Anti-GBM is an indirect solid phase enzyme immunoassay (ELISA) for the quantitative measurement of IgG class autoantibodies to glomerular basement membrane (GBM) in human serum or plasma. |
| Detection Method | Colorimetric |
| Includes | 1.5mL Anti-GBM calibrator, 1.5mL Anti-GBM controls, 20mL Sample Buffer, 15mL Enzyme Conjugate, 15mL TMB Substrate solution, 15mL stop solution, 12 micro titer strips |
| Quantity | 1 Kit |
| Sample Type | Plasma, Serum |
| Sample Volume | 100 μL |
| Content And Storage | Store the kit at 4°C |
| For Use With (Application) | Quantification |
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