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Abnova™ GBM Ab ELISA Kit

Indirect solid phase Enzyme Immunoassay (ELISA) for the quantitative measurement of IgG class autoantibodies to glomerular basement membrane (GBM) in Human Serum or Plasma

Manufacturer:  Abnova™ KA1266

Catalog No. 89-024-558


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Includes:
  • Micro titer strips Divisible microplate consisting of 12 modules of 8 wells each, coated with highly purified GBM. Ready to use. 12
  • Anti-GBM Calibrators (A-E) in a serum/buffer matrix (PBS, BSA, NaN3 <0.1% (w/w)) containing: 0; 20; 40; 80; 200U/mL Ready to use. 5 vials, 1.5mL each
  • Anti-GBM Controls in a serum/buffer matrix (PBS, BSA, NaN3 <0.1% (w/w)) positive (1) and negative (2), for the respective concentrations see the enclosed package insert. Ready to use. 2 vials, 1.5mL each
  • Sample buffer (Tris, NaN3 <0.1% (w/w)), yellow, concentrate (5x). 1 vial, 20mL
  • Enzyme Conjugate (PBS, Proclin 300 <0.5% (v/v)), (light red) containing polyclonal rabbit anti-human IgG; labelled with horseradish peroxidase. Ready to use. 1 vial, 15mL
  • TMB substrate solution TMB substrate solution. Ready to use. 1 vial, 15mL
  • Wash solution (PBS, NaN3 <0.1% (w/w)), concentrate (50x). 1 vial, 15mL

Specifications

Specifications

HRP
Anti-GBM is an indirect solid phase enzyme immunoassay (ELISA) for the quantitative measurement of IgG class autoantibodies to glomerular basement membrane (GBM) in human serum or plasma.
Quantification
GBM Ab ELISA Kit
1 Kit
Store the kit at 4°C.
Human
Colorimetric
1.5mL Anti-GBM calibrator, 1.5mL Anti-GBM controls, 20mL Sample Buffer, 15mL Enzyme Conjugate, 15mL TMB Substrate solution, 15mL stop solution, 12 micro titer strips
GBM Ab ELISA kit
Plasma, Serum
100μL
Documents
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