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Abnova™ Ghrelin (Human/Mouse/Rat) ELISA Kit

Catalog No. 89028574 Shop All Abnova Corporation Products
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Catalog No. Quantity
89-028-574 1 Kit
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Catalog No. 89-028-574 Supplier Abnova™ Supplier No. KA1863

Description

Includes

  • Ghrelin microplate (Item A): Coated with secondary antibody. 96 wells (12 strips x 8 wells)
  • Wash buffer concentrate (20X) (Item B) 25mL
  • Standard Ghrelin peptide (Item C) 2 vials, 10μL/vial
  • Anti- Ghrelin polyclonal antibody (Item N) 2 vials, 5μL/vial
  • Assay diluent A (Item D): contains 0.09% sodium azide as preservative.
  • For Standard/Sample (serum/ plasma) diluent, 30mL
  • Assay diluent B (Item E): 5X concentrated buffer. Diluent for standards and cell culture media or other sample types, 15mL
  • Biotinylated Ghrelin peptide, (Item F) 2 vials, 20μL/vial
  • HRP-Streptavidin concentrate (Item G): 200X concentrated HRP-conjugated Streptavidin, 600μL
  • Positive control (Item M) 1 vial, 100μL
  • TMB one-step substrate reagent (Item H): 3, 3', 5, 5'- tetramethylbenzidine (TMB) in buffered solution, 12mL
  • Stop solution (Item I): 0.2M sulfuric acid, 8mL

In-vitro quantitative assay for detecting Ghrelin based on the principle of competitive Enzyme Immunoassay

Specifications

Calibration Range 0.1 to 1000ng/mL
Conjugate Biotin
Cross Reactivity Human, Mouse, Rat
Description Ghrelin (Human, Mouse, Rat) ELISA Kit is an in vitro quantitative assay for detecting Ghrelin based on the principle of Competitive Enzyme Immunoassay.
Detection Limit 161pg/mL
Detection Method Colorimetric
Includes 96-well Antibody coated microwell, 25mL Wash Buffer (20X), 2 vials detected Polyclonal Antibody, 2 vials standard, 30mL Assay diluent A, 15mL Assay diluent B, 2 vials Biotinylated peptide, 8μL HRP Streptavidian concentrate, 100
Quantity 1 Kit
Sample Type Cell Culture Supernatant, Cell Lysate, Plasma, Serum
Sample Volume 100 μL
Content And Storage Store the kit at -20°C. For best results, please store the components as described in the protocol. Avoid repeated freezing and thawing.
For Use With (Application) Quantification
Product Type Ghrelin (Human/Mouse/Rat) ELISA kit
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