Abnova™ Ghrelin (Human/Mouse/Rat) ELISA Kit

In-vitro quantitative assay for detecting Ghrelin based on the principle of competitive Enzyme Immunoassay

Manufacturer:  Abnova™ KA1863

Catalog No. 89-028-574

Includes:

• Ghrelin microplate (Item A): Coated with secondary antibody. 96 wells (12 strips x 8 wells)
• Wash buffer concentrate (20X) (Item B) 25mL
• Standard Ghrelin peptide (Item C) 2 vials, 10µL/vial
• Anti- Ghrelin polyclonal antibody (Item N) 2 vials, 5µL/vial
• Assay diluent A (Item D): contains 0.09% sodium azide as preservative.
• For Standard/Sample (serum/ plasma) diluent, 30mL
• Assay diluent B (Item E): 5X concentrated buffer. Diluent for standards and cell culture media or other sample types, 15mL
• Biotinylated Ghrelin peptide, (Item F) 2 vials, 20µL/vial
• HRP-Streptavidin concentrate (Item G): 200X concentrated HRP-conjugated Streptavidin, 600µL
• Positive control (Item M) 1 vial, 100µL
• TMB one-step substrate reagent (Item H): 3, 3', 5, 5'- tetramethylbenzidine (TMB) in buffered solution, 12mL
• Stop solution (Item I): 0.2M sulfuric acid, 8mL

Specifications

• Calibration Range: 0.1 to 1000ng/mL
• Cross Reactivity: Human,Mouse,Rat
• Detection Limit: 161pg/mL
• For Use With (Application): Quantification
• Product Name: Ghrelin (Human/Mouse/Rat) ELISA Kit
• Quantity: 1 Kit
• Storage Requirements: Store the kit at -20°C. For best results, please store the components as described in the protocol. Avoid repeated freezing and thawing.

• Conjugate: Biotin
• Description: Ghrelin (Human, Mouse, Rat) ELISA Kit is an in vitro quantitative assay for detecting Ghrelin based on the principle of Competitive Enzyme Immunoassay.
• Detection Method: Colorimetric
• Includes: 96-well Antibody coated microwell, 25mL Wash Buffer (20X), 2 vials detected Polyclonal Antibody, 2 vials standard, 30mL Assay diluent A, 15mL Assay diluent B, 2 vials Biotinylated peptide, 8μL HRP Streptavidian concentrate, 100
• Product Type: Ghrelin (Human/Mouse/Rat) ELISA kit
• Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum
• Volume (Metric) Sample: 100μL