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Abnova™ Ghrelin (Human/Mouse/Rat) ELISA Kit

In-vitro quantitative assay for detecting Ghrelin based on the principle of competitive Enzyme Immunoassay

Manufacturer:  Abnova™ KA1863

Catalog No. 89-028-574

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Includes:
  • Ghrelin microplate (Item A): Coated with secondary antibody. 96 wells (12 strips x 8 wells)
  • Wash buffer concentrate (20X) (Item B) 25mL
  • Standard Ghrelin peptide (Item C) 2 vials, 10µL/vial
  • Anti- Ghrelin polyclonal antibody (Item N) 2 vials, 5µL/vial
  • Assay diluent A (Item D): contains 0.09% sodium azide as preservative.
  • For Standard/Sample (serum/ plasma) diluent, 30mL
  • Assay diluent B (Item E): 5X concentrated buffer. Diluent for standards and cell culture media or other sample types, 15mL
  • Biotinylated Ghrelin peptide, (Item F) 2 vials, 20µL/vial
  • HRP-Streptavidin concentrate (Item G): 200X concentrated HRP-conjugated Streptavidin, 600µL
  • Positive control (Item M) 1 vial, 100µL
  • TMB one-step substrate reagent (Item H): 3, 3', 5, 5'- tetramethylbenzidine (TMB) in buffered solution, 12mL
  • Stop solution (Item I): 0.2M sulfuric acid, 8mL

Specifications

Specifications

0.1 to 1000ng/mL
Human,Mouse,Rat
161pg/mL
Quantification
Ghrelin (Human/Mouse/Rat) ELISA Kit
1 Kit
Store the kit at -20°C. For best results, please store the components as described in the protocol. Avoid repeated freezing and thawing.
Biotin
Ghrelin (Human, Mouse, Rat) ELISA Kit is an in vitro quantitative assay for detecting Ghrelin based on the principle of Competitive Enzyme Immunoassay.
Colorimetric
96-well Antibody coated microwell, 25mL Wash Buffer (20X), 2 vials detected Polyclonal Antibody, 2 vials standard, 30mL Assay diluent A, 15mL Assay diluent B, 2 vials Biotinylated peptide, 8μL HRP Streptavidian concentrate, 100
Ghrelin (Human/Mouse/Rat) ELISA kit
Cell Culture Supernatant, Cell Lysate, Plasma, Serum
100μL
Documents
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