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Abnova™ Histone H3 (Total) Quantification Kit (Fluorometric)

Histone H3 (Total) Quantification Kit (Fluorometric) is designed for quantifying levels of histone H3 proteins independent of its modified state and can also be used for normalizing the modified histone H3 content of samples when run in parallel with other histone modification quantification kit series.

Manufacturer:  Abnova™ KA0625

Catalog No. 89-027-186


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Includes:
  • F1 (10X wash buffer) 20mL
  • F2 (antibody buffer) 12mL
  • F3 (detection antibody, 1mg/ml) 10µL
  • F4 (fluoro developer) 24µL
  • F5 (fluoro enhancer) 24µL
  • F6 (fluoro dilution) 8mL
  • Standard control (100µg/ml) 20µL
  • 8 well sample strips (with frame) 9
  • 8 well standard control strips 3

Description

Description

  • Quick and efficient procedure which can be finished within 2.5 hours
  • Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography
  • Specifically captures histone H3 with the detection limit as low as 2 ng/well and detection range from 10 ng-1 µg/well of histone extracts
  • The unmodified histone H3 control is conveniently included for quantification of the amount of total histone H3
  • Strip microplate format makes the assay flexible, manual or high throughput
  • Simple, reliable, and consistent assay conditions
  • Kit is shipped in two parts, one at ambient room temperature and the second on frozen ice packs at 4°C
  • Kit configured for research use only and is not intended for diagnostic and therapeutic application

Specifications

Specifications

96 Assays
Quantification
Store F3, F4 and Standard control at -20°C. Store all other components at 4°C away from light.
Histone H3 (Total) Quantification Kit (Fluorometric) is designed for quantifying levels of histone H3 proteins independent of its modified state and can also be used for normalizing the modified histone H3 content of samples when run in parallel with other histone modification quantification kit series.
Histo ne H3 (to tal) quantification Kit
Fluorometric
Documents
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