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Description
Includes
- Mek1 Microplate: 96 wells coated with anti-pan Mek1 antibody
- Wash Buffer Concentrate (20x)
- Assay Diluent: 15mL of 5x concentrated buffer. For diluting cell lysate sample, detection antibody and HRP-conjugated anti-rabbit IgG concentrate
- Detection Antibody Mek1 (Ser217/221): rabbit anti-Mek1 (Ser217/221)
- HRP-conjugated Anti-rabbit IgG, 25μL of 500x HRP-conjugated anti-rabbit IgG concentrate
- TMB One-Step Substrate Reagent
- Stop Solution: 2M sulfuric acid
- Cell Lysate Buffer: 5mL 2x cell lysis buffer (not including protease and phosphatase inhibitors)
- Positive Control HelaT003-1: lyophilized powder from treated Hela cell lysate
Specifications
Specifications
| Conjugate | HRP |
| Cross Reactivity | Human, Mouse, Rat |
| Description | MAP2K1 (pS217/221) (Human/Mouse/Rat) ELISA Kit is an enzyme-linked immunosorbent assay for the qualitative determination of human, mouse, rat phospho MAP2K1 (S217/221). |
| Detection Method | Colorimetric |
| Quantity | 1 Kit |
| Regulatory Status | For research use only (RUO) |
| Sample Type | Cell Lysate, Tissue Lysate |
| Sample Volume | 100 μL |
| Packaging | 96-well |
| Content And Storage | Store the kit at -20°C |
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