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Abnova™ NPPB (Human/Mouse/Rat) ELISA Kit

Catalog No. 89028572 Shop All Abnova Corporation Products
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Catalog No. Quantity
89-028-572 1 Kit
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Catalog No. 89-028-572 Supplier Abnova™ Supplier No. KA1861

Description

Includes

  • BNP microplate (Item A): coated with secondary antibody 96 (8 x 12) wells
  • Wash buffer concentrate (20X) (Item B) 25mL
  • Lyophilized standard BNP Peptide (Item C), 2 vials
  • Lyophilized anti- BNP polyclonal antibody (Item N), 2 vials
  • 1X assay diluent E (Item R): Diluent for both standards and samples including serum, plasma, cell culture media or other sample types, 25mL x 2
  • Lyophilized biotinylated BNP peptide (Item F), 2 vials
  • HRP-Streptavidin concentrate (Item G): 200X concentrated HRP-conjugated Streptavidin, 600μL
  • Lyophilized positive control (Item M), 1 vial
  • TMB One-Step Substrate Reagent (Item H): 3, 3', 5, 5'-tetramethylbenzidine (TMB) in buffered solution, 12mL
  • Stop solution (Item I): 0.2M sulfuric acid, 8mL

For detecting NPPB/Nppb based on the principle of competitive Enzyme Immunoassay

Specifications

Calibration Range 0.1 to 1000pg/mL
Conjugate Biotin
Cross Reactivity Human, Mouse, Rat
Description NPPB/Nppb (Human, Mouse, Rat) ELISA Kit is an in vitro quantitative assay for detecting NPPB/Nppb based on the principle of Competitive Enzyme Immunoassay.
Detection Limit 1.02pg/mL
Detection Method Colorimetric
Includes 96-well Antibody coated microwell, 25mL Wash Buffer 20X, detected Polyclonal Antibody 2 vials, standard 2 vials, 30mL assay diluent A, 15mL assay diluent B, Biotinylated peptide 2 vials, 8μL HRP-Streptavidian concentrate, 100id=
Quantity 1 Kit
Sample Type Cell Culture Supernatant, Cell Lysate, Plasma, Serum
Sample Volume 100 μL
Content And Storage Store the kit at -20°C. For best results, please store the components as described in the protocol. Avoid repeated freezing and thawing.
For Use With (Application) Quantification
Product Type NPPB (Human/Mouse/Rat) ELISA kit
Specificity This kit detects both BNP-32 (mature form), pro-BNP (immature form), and possible other forms of BNP, but not NT-pro BNP.
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