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Description
Includes
- rBNP-45 Microplate: A 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody against rBNP-45, 1 in no.
- Sealing Tapes: pressure-sensitive sealing tapes, which can be cut to fit the format of the individual assay, 3 in nos.
- rBNP45 Standard: Rat BNP-45 in a buffered protein base (lyophilized), 4ng
- Biotinylated rBNP-45 Antibody (100x): A 100-fold biotinylated polyclonal antibody against rBNP-45, 80μL
- MIX Diluent Concentrate (10x): A 10-fold buffered protein base, 30mL
- Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant, 30mL, 2 bottles
- Streptavidin-Peroxidase Conjugate: A 100-fold concentrate 80μL
- Ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine, 8mL
- Stop Solution: A 0.5N hydroxychloric acid to stop the chromogen substrate reaction, 12mL
Specifications
Specifications
| Calibration Range | 0.031 to 1ng/mL |
| Conjugate | Biotin |
| Description | Nppb (Rat) ELISA Kit is designed for detection of rat Nppb in rat plasma, serum, tissue extract, and cell culture supernatants. |
| Detection Limit | 0.03ng/mL |
| Detection Method | Colorimetric |
| Includes | 96-well Antibody coated microwell, 3ng rBNP45 standard, 100X Biotinylated rBNP45 Antibody 1 vial, 30mL MIX diluent concentrate (10X), Wash Buffer 20X 30mL x 2, Streptavidin-peroxidase Conjugate 80, 8mL Chromogen Substrate, 12mL stop solution |
| Quantity | 96 Assays |
| Sample Type | CCS, Plasma, Serum, Tissue |
| Sample Volume | 50 μL |
| Content And Storage | Store components of the kit at 4°C or -20°C as described in the protocol. |
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