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Description
Includes
- PF-4 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human PF-4, 96-wells (12 strips x 8 wells)
- Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution, 25mL
- Standards (Item C): recombinant human PF-4, 2 vials
- Assay Diluent C (Item L): diluents buffer. For Standard/Sample (serum/plasma samples/cell culture medium/urine) diluent, 30mL
- Assay Diluent B (Item E): 5x concentrated buffer. For detection antibody and HRP- Streptavidin diluent, 15mL
- Detection Antibody PF-4 (Item F): biotinylated anti-human PF-4 (each vial is enough to assay half microplate), 2 vials
- HRP-Streptavidin concentrates (Item G): 500x concentrated, HRP-conjugated Streptavidin, 200μLl
- TMB One-Step Substrate Reagent (Item H): of 3, 3', 5, 5'- tetramethylbenzidine (TMB) in buffered solution, 12mL
- Stop Solution (Item I): 0.2M sulfuric acid 8mL
Specifications
Specifications
| Conjugate | Biotin |
| Cross Reactivity | Human |
| Description | PF4 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PF4. |
| Detection Limit | 20pg/mL |
| Detection Method | Colorimetric |
| Includes | 96-well Antibody coated microwell, 25mL Wash Buffer 20X, standard 2 vials, 30mL assay diluent A, 15mL assay diluent B, detection Antibody MSP 2 vials, 8μL HRP-Streptavidian concentrate, 12mL TMB One-Step Substrate reagent, 8mL stop solut |
| Quantity | 96 Assays |
| Sample Type | Cell Culture Supernatant, Plasma, Serum, Urine |
| Sample Volume | 100 μL |
| Content And Storage | Store the kit at -20°C. For best results, please store the components as described in the protocol. Avoid repeated freezing and thawing. |
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