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Abnova™ PF4 (Human) ELISA Kit

In-Vitro Enzyme-Linked Immunosorbent assay for the quantitative measurement of Human PF4

Manufacturer:  Abnova™ KA1761

Catalog No. 89-028-496

This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.


Includes:
  • PF-4 Microplate (Item A): 96 wells (12 strips x 8 wells) coated with anti-human PF-4, 96-wells (12 strips x 8 wells)
  • Wash Buffer Concentrate (20x) (Item B): 20x concentrated solution, 25mL
  • Standards (Item C): recombinant human PF-4, 2 vials
  • Assay Diluent C (Item L): diluents buffer. For Standard/Sample (serum/plasma samples/cell culture medium/urine) diluent, 30mL
  • Assay Diluent B (Item E): 5x concentrated buffer. For detection antibody and HRP- Streptavidin diluent, 15mL
  • Detection Antibody PF-4 (Item F): biotinylated anti-human PF-4 (each vial is enough to assay half microplate), 2 vials
  • HRP-Streptavidin concentrates (Item G): 500x concentrated, HRP-conjugated Streptavidin, 200µLl
  • TMB One-Step Substrate Reagent (Item H): of 3, 3', 5, 5'- tetramethylbenzidine (TMB) in buffered solution, 12mL
  • Stop Solution (Item I): 0.2M sulfuric acid 8mL

Specifications

Specifications

Biotin
PF4 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PF4.
Colorimetric
96-well Antibody coated microwell, 25mL Wash Buffer 20X, standard 2 vials, 30mL assay diluent A, 15mL assay diluent B, detection Antibody MSP 2 vials, 8μL HRP-Streptavidian concentrate, 12mL TMB One-Step Substrate reagent, 8mL stop solut
96 Assays
Store the kit at -20°C. For best results, please store the components as described in the protocol. Avoid repeated freezing and thawing.
Human
20pg/mL
Quantification
PF4 (Human) ELISA Kit
Cell Culture Supernatant, Plasma, Serum, Urine
100μL
Documents
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