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Description
Includes
- Human tPA Microplate: A 96 well polystyrene microplate coated with a polyclonal antibody against tPA. 96 wells (12 strips of 8 wells)
- Sealing Tapes: Each kit contains 3 precut, pressure sensitive sealing tapes that can be cut to fit the format of the individual assay. 3 Human tPA Standard: Human tPA in a buffered protein base (lyophilized). 10ng
- Biotinylated Human tPA antibody (50x): A 50-fold concentrated biotinylated antibody against human tPA. 140μL
- MIX Diluent Concentrate (10x): A 10-fold concentrated buffered protein base. 30mL
- Wash Buffer Concentrate (20x): A 20-fold concentrated buffered surfactant. 30mL x 2
- Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate. 80μL
- Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine. 8mL
- Stop Solution: A 0.5 N hydrochloric acid to stop the chromogen substrate reaction. 12mL
- Kit is designed for detection of human tPA in plasma, serum, milk, cell culture supernatants, and tissue extract
- This assay employs a quantitative sandwich enzyme immunoassay technique that measures total tPA in less than 4 hours
- Store components of the kit at 2-8°C or -20°C upon arrival to the expiration date
- For research use only
Specifications
Specifications
| Conjugate | Biotin |
| Cross Reactivity | Human, Pig |
| Description | PLAT (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human PLAT. |
| Detection Limit | 0.1 ng/mL |
| Detection Method | Colorimetric |
| Quantity | 1 Kit |
| Regulatory Status | For research use only (RUO) |
| Sample Type | Cell culture supernatant, milk, plasma, serum and tissue extract |
| Sample Volume | 50 μL |
| Content And Storage | Store the kit at 4°C. For long term storage, please store at -20°C. |
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