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  5. Abnova™ SERPINA1 (Human) ELISA Kit

Abnova™ SERPINA1 (Human) ELISA Kit

Catalog No. 89100251 Shop All Abnova Corporation Products
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Calibration Range:
0.039 to 40 μg/mL
0.391 to 100 ng/mL
Detection Limit:
0.018 μg/mL
0.16 ng/mL
Sample Volume:
25 μL
50 μL
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Catalog No. Calibration Range Detection Limit Sample Volume
89-100-251 0.391 to 100 ng/mL 0.16 ng/mL 50 μL
89-100-252 0.039 to 40 μg/mL 0.018 μg/mL 25 μL
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Catalog No. 89-100-251 Supplier Abnova™ Supplier No. KA0459
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Description

Includes

  • Human A1AT Microplate: A polystyrene microplate coated with a polyclonal antibody against human A1AT
  • Sealing Tapes
  • Human A1AT Standard: Human A1AT in a buffered protein base, lyophilized, 150ng
  • Biotinylated Human A1AT Antibody (50x): A 50-fold biotinylated polyclonal antibody against human A1AT, 120μL
  • MIX Diluent Concentrate (10x)
  • Wash Buffer Concentrate (20x)
  • Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate, 80μL
  • Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine
  • Stop Solution: A 0.5N hydrochloric acid

This assay employs a quantitative sandwich enzyme immunoassay technique that measures A1AT in less than 4 hours

Specifications

Calibration Range 0.391 to 100 ng/mL
Conjugate Biotin
Cross Reactivity Human
Description SERPINA1 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human SERPINA1 in cell culture supernatant.
Detection Limit 0.16 ng/mL
Detection Method Colorimetric
Includes Antibody coated microwells (96 wells), Human A1AT Standard (400ng), Biotinylated A1AT Antibody (100x) (1 vial), MIX Diluent Concentrate (10x) (30mL), Wash Buffer Concentrate (20x) (30mL x 2), Streptavidin-Peroxidase Conjugate (SP Conjugate) 80, Chromogen Substrate (8mL), Stop Solution: (12mL)
Quantity 1 Kit
Regulatory Status For research use only (RUO)
Sample Type Cell Culture Samples, CSF, Milk, Saliva, Urine
Sample Volume 50 μL
Packaging 96-well
Content And Storage Store components of the kit at 4°C or -20°C as described in the protocol.
For Use With (Application) Quantification
Product Type ELISA Kit
No. of Assays 96
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