Abnova™ SERPINA1 (Human) ELISA Kit

Description

Includes

• Human A1AT Microplate: A polystyrene microplate coated with a polyclonal antibody against human A1AT
• Sealing Tapes
• Human A1AT Standard: Human A1AT in a buffered protein base, lyophilized, 150ng
• Biotinylated Human A1AT Antibody (50x): A 50-fold biotinylated polyclonal antibody against human A1AT, 120μL
• MIX Diluent Concentrate (10x)
• Wash Buffer Concentrate (20x)
• Streptavidin-Peroxidase Conjugate (SP Conjugate): A 100-fold concentrate, 80μL
• Chromogen Substrate: A ready-to-use stabilized peroxidase chromogen substrate tetramethylbenzidine
• Stop Solution: A 0.5N hydrochloric acid

This assay employs a quantitative sandwich enzyme immunoassay technique that measures A1AT in less than 4 hours

Specifications

• Calibration Range: 0.391 to 100 ng/mL
• Conjugate: Biotin
• Cross Reactivity: Human
• Description: SERPINA1 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human SERPINA1 in cell culture supernatant.
• Detection Limit: 0.16 ng/mL
• Detection Method: Colorimetric
• Includes: Antibody coated microwells (96 wells), Human A1AT Standard (400ng), Biotinylated A1AT Antibody (100x) (1 vial), MIX Diluent Concentrate (10x) (30mL), Wash Buffer Concentrate (20x) (30mL x 2), Streptavidin-Peroxidase Conjugate (SP Conjugate) 80, Chromogen Substrate (8mL), Stop Solution: (12mL)
• Quantity: 1 Kit
• Regulatory Status: For research use only (RUO)
• Sample Type: Cell Culture Samples, CSF, Milk, Saliva, Urine
• Sample Volume: 50 μL
• Packaging: 96-well
• Content And Storage: Store components of the kit at 4°C or -20°C as described in the protocol.
• For Use With (Application): Quantification
• Product Type: ELISA Kit
• No. of Assays: 96