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Description
Includes
- Shh-N Microplate (Item A): 96 wells coated with anti-human Shh-N
- Wash Buffer Concentrate (20x) (Item B)
- Standards (Item C): Recombinant human Shh-N
- Assay Diluent C (Item L): Diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
- Assay Diluent B (Item E): 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
- Detection Antibody (Item F): Biotinylated anti-human Shh-N
- HRP-Streptavidin Concentrate (Item G): 200x concentrated HRP-conjugated streptavidin
- TMB One-Step Substrate Reagent (Item H)
- Stop Solution (Item I): 0.2M sulfuric acid
Specifications
Specifications
| Conjugate | HRP |
| Cross Reactivity | Human |
| Description | SHH (N-terminal) (Human) ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of human SHH N-terminal. |
| Detection Limit | 42pg/mL |
| Detection Method | Colorimetric |
| Quantity | 1 Kit |
| Regulatory Status | For research use only (RUO) |
| Sample Type | Serum, Plasma, Cell Culture Supernatant, Urine |
| Sample Volume | 100 μL |
| Sensitivity | The minimum detectable dose of Shh to N is typically less than 42 pg/mL in Assay Diluent C and 8 pg/mL in Assay Diluent B respectivelly |
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