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Abnova™ SREBF2 Transcription Factor Assay Kit

Catalog No. 89024653 Shop All Abnova Corporation Products
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Catalog No. 89-024-653 Supplier Abnova™ Supplier No. KA1379
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SREBF2 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.

  • A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA)
  • A specific double stranded DNA (dsDNA) sequence containing the SREBP response element is immobilized onto the wells of a 96-well plate
  • SREBP contained in a nuclear extract, binds specifically to the SREBP response element
  • SREBP is detected by addition of a specific primary antibody directed against SREBP
  • A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm
  • This product is for laboratory research use only: not for administration to humans
  • This kit will perform as specified if stored as directed and used before the expiration date indicated on the outside of the box

Specifications

Description SREBF2 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.
Immunoassay Kit Format ELISA (Standard)
Product Type SREBF2 Transcription Factor Assay Kit
Sufficient For 15 Reactions
For Use With (Application) Functional Study
Gene ID (Entrez) 6721
Gene Symbol SREBF2
Kit Contents Transcription factor antibody binding buffer (10X), Transcription factor binding assay buffer (4X), Transcription factor reagent A, Transcription factor SREBP-2 positive control, Transcription factor SREBP-2 primary antibody, Wash buffer concentrate
Quantity 1 Kit
Storage Requirements Store the kit at -20°C temporarily. For best results, store the components as described in the protocol.
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