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Description
- A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA)
- A specific double stranded DNA (dsDNA) sequence containing the SREBP response element is immobilized onto the wells of a 96-well plate
- SREBP contained in a nuclear extract, binds specifically to the SREBP response element
- SREBP is detected by addition of a specific primary antibody directed against SREBP
- A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm
- This product is for laboratory research use only: not for administration to humans
- This kit will perform as specified if stored as directed and used before the expiration date indicated on the outside of the box
Specifications
Specifications
| Description | SREBF2 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. |
| Immunoassay Kit Format | ELISA (Standard) |
| Product Type | SREBF2 Transcription Factor Assay Kit |
| Sufficient For | 15 Reactions |
| For Use With (Application) | Functional Study |
| Gene ID (Entrez) | 6721 |
| Gene Symbol | SREBF2 |
| Kit Contents | Transcription factor antibody binding buffer (10X), Transcription factor binding assay buffer (4X), Transcription factor reagent A, Transcription factor SREBP-2 positive control, Transcription factor SREBP-2 primary antibody, Wash buffer concentrate |
| Quantity | 1 Kit |
| Storage Requirements | Store the kit at -20°C temporarily. For best results, store the components as described in the protocol. |
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