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Abnova™ SREBF2 Transcription Factor Assay Kit

SREBF2 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.

Manufacturer:  Abnova™ KA1379

Catalog No. 89-024-653


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Description

Description

  • A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA)
  • A specific double stranded DNA (dsDNA) sequence containing the SREBP response element is immobilized onto the wells of a 96-well plate
  • SREBP contained in a nuclear extract, binds specifically to the SREBP response element
  • SREBP is detected by addition of a specific primary antibody directed against SREBP
  • A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm
  • This product is for laboratory research use only: not for administration to humans
  • This kit will perform as specified if stored as directed and used before the expiration date indicated on the outside of the box

Specifications

Specifications

SREBF2 Transcription Factor Assay Kit is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates.
Functional Study
Transcription factor SREBP-2 positive control
Store the kit at -20°C temporarily. For best results, store the components as described in the protocol.
SREBF2
Solution
6721
SREBF2 Transcription Factor Assay Kit
15 Reactions
1 Kit
Documents
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