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Abnova™ TNF (Human) ELISA Kit

Catalog No. 89100815 Shop All Abnova Corporation Products
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96 Assays
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89-100-815 96 Assays
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Catalog No. 89-100-815 Supplier Abnova™ Supplier No. KA3113
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Description

Sandwich Enzyme Immunoassay for the quantitative measurement of Human TNF

Specifications

Calibration Range 15.6 to 1000pg/mL (Body Fluid, Tissue Lysate, Cell Culture Supernatant), 7.8 to 500pg/mL (Serum)
Conjugate Biotin
Cross Reactivity Human
Description TNF (Human) ELISA Kit is intended for the quantitative measurement of human TNF.
Detection Limit 1pg/mL
Detection Method Colorimetric
Includes One 96-well plate pre-coated with anti-Human TNF-alpha antibody, Lyophilized Human TNFα standards: 10ng x 2, Sample / Standard diluent buffer: 30mL, Biotin conjugated anti-Human TNFα antibody (Concentrated Dilution: 1:100): 130μL, Antibody diluent buffer: 12mL, Avidin-Biotin-Peroxidase Complex (ABC) (ConcentratedDilution: 1:100): 130μL, ABC diluent buffer: 12mL, TMB substrate: 10mL, Stop solution: 10mL, Wash buffer (25X): 30mL
Quantity 96 Assays
Sample Type Body Fluid, Cell Culture Supernate, Serum, Tissue Lysate
Sample Volume 100 μL
Content And Storage Store at 4°C for 4 months, or at -20°C for 8 months.
For Use With (Application) Quantification
Product Type TNF (Human) ELISA Kit
No. of Assays 96
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