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Abnova™ TNF (Mouse) ELISA Kit

Catalog No. 89100816
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Quantity:
1 Kit

Includes

  • One 96-well plate pre-coated with anti-Mouse TNFα antibody, 96 (8 x 12) wells
  • Lyophilized Mouse TNFα standards, 10ng x 2
  • Sample/Standard diluent buffer, 30mL
  • Biotin conjugated anti-Mouse TNFα antibody (Concentrated) Dilution: 1:100, 130μL
  • Antibody diluent buffer, 12mL
  • Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated) Dilution: 1:100, 130μL
  • ABC diluent buffer, 12mL
  • TMB substrate, 10mL
  • Stop solution, 10mL
  • Wash buffer (25X), 30mL

Use to measure quantitatively mouse Tnf.

This kit is based on sandwich enzyme-linked immune-sorbent assay technology

Specifications

Calibration Range 15.6 to 1000pg/mL (Body Fluid, Tissue Lysate, Cell Culture Supernatant), 7.8 to 500pg/mL (Serum, Plasma)
Conjugate Biotin
Cross Reactivity Mouse
Description Tnf (Mouse) ELISA Kit is intended for the quantitative measurement of mouse Tnf.
Detection Limit 1pg/mL
Detection Method Colorimetric
Includes One 96-well plate pre-coated with anti-Mouse TNFα antibody 96 (8x12) wells, Lyophilized TNFα standards (10ng x 2), Sample / Standard diluent buffer (30mL), Biotin conjugated anti-Mouse TNFα antibody (Concentrated), Dilution: 1:100 (130μL) Antibody diluent buffer (12mL), Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated), Dilution: 1:100 (130μL), ABC diluent buffer (12mL), TMB substrate (10mL), Stop solution (10mL), Wash buffer (25X) (30mL)
Quantity 1 Kit
Regulatory Status For research use only (RUO)
Sample Type Body Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate
Sample Volume 100 μL
Sensitivity <1 pg/mL
Packaging 96-well
Content And Storage Store at 4°C for 4 months, or at -20°C for 8 months.
For Use With (Application) Quantification
Product Type Tnf (Mouse) ELISA Kit
No. of Assays 96
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