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Abnova™ TNF (Rat) ELISA Kit

Catalog No. 89100817 Shop All Abnova Corporation Products
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Catalog No. Quantity
89-100-817 1 Kit
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Catalog No. 89-100-817 Supplier Abnova™ Supplier No. KA3115
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Includes

  • One 96-well plate pre-coated with anti-Rat TNFα antibody, 96 (8 x 12) wells
  • Lyophilized TNFα standards, 10ng x 2
  • Sample/Standard diluent buffer, 30mL
  • Biotin conjugated anti-Rat TNFα antibody (Concentrated), Dilution: 1:100, 130μL
  • Antibody diluent buffer, 12mL
  • Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated), Dilution: 1:100, 130μL
  • ABC diluent buffer, 12mL
  • TMB substrate, 10mL
  • Stop solution, 10mL
  • Wash buffer (25X), 30mL

Use to measure quantitatively rat Tnf.

This kit is based on sandwich enzyme-linked immune-sorbent assay technology

Specifications

Calibration Range 15.6 to 1000pg/mL (Body Fluid, Tissue Lysate, Cell Culture Supernatant), 7.8 to 501pg/mL (Serum)
Conjugate Biotin
Cross Reactivity Rat
Description Tnf (Rat) ELISA Kit is intended for the quantitative measurement of rat Tnf.
Detection Limit 1pg/mL
Detection Method Colorimetric
Includes One 96-well plate pre-coated with anti-Rat TNFα antibody 96 (8x12) wells, Lyophilized anti-Rat TNFα standards (10ng x 2), Sample / Standard diluent buffer (30mL), Biotin conjugated anti-Rat TNFα antibody (Concentrated), Dilution: 1:100 (130μL) Antibody diluent buffer (12mL), Avidin-Biotin-Peroxidase Complex (ABC) (Concentrated), Dilution: 1:100 (130μL), ABC diluent buffer (12mL), TMB substrate (10mL), Stop solution (10mL), Wash buffer (25X) (30mL)
Quantity 1 Kit
Regulatory Status For research use only (RUO)
Sample Type Body Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate
Sample Volume 100 μL
Sensitivity <1 pg/mL
Packaging 96-well
Content And Storage Store at 4°C for 4 months, or at -20°C for 8 months.
For Use With (Application) Quantification
Product Type Tnf (Rat) ELISA Kit
No. of Assays 96
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