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Enzo Life Sciences Ac-DEVD-AFC (5 mg) CAS: 201608-14-2
SDP

Catalog No. NC0664508
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Caspase-3 substrate. Fluorogenic substrate for caspase-3 (CPP32), with a Km=9.7µM) and related cysteine proteases. Sequence is based on PARP cleavage at Asp216 for caspase-3. Similar to Ac-DEVD-AMC (Prod. No. ALX-260-031) but the AFC fluorophore has a greater Stokes’ shift upon cleavage than AMC. Reaction can be monitored quantitatively or visually using a hand-held long-UV lamp and visualizing a blue to green shift in fluorescence upon cleavage. Ex.: 400nm, Em.: 505nm. Alternative Name: Caspase-3 substrate (fluorogenic). Formula: C30H34F3N5O13. MW: 729.6. CAS: 201608-14-2. Purity: ≥96% (HPLC). Sequence Ac-Asp-Glu-Val-Asp-AFC (AFC = 7-Amino-4-trifluoromethylcoumarin). Appearance: White to off-white powder. Solubility: Soluble in dimethyl formamide, DMSO or methanol; slightly soluble in water (0.4mg/ml). Long Term Storage: -20°C. Handling: Protect from light. Keep cool and dry.

Catalog No. NC0664508 Supplier Enzo Life Sciences Supplier No. ALX260032M005
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Caspase-3 substrate. Fluorogenic substrate for caspase-3 (CPP32), with a Km=9.7µM) and related cysteine proteases. Sequence is based on PARP cleavage at Asp216 for caspase-3. Similar to Ac-DEVD-AMC (Prod. No. ALX-260-031) but the AFC fluorophore has a greater Stokes’ shift upon cleavage than AMC. Reaction can be monitored quantitatively or visually using a hand-held long-UV lamp and visualizing a blue to green shift in fluorescence upon cleavage. Ex.: 400nm, Em.: 505nm. Alternative Name: Caspase-3 substrate (fluorogenic). Formula: C30H34F3N5O13. MW: 729.6. CAS: 201608-14-2. Purity: ≥96% (HPLC). Sequence Ac-Asp-Glu-Val-Asp-AFC (AFC = 7-Amino-4-trifluoromethylcoumarin). Appearance: White to off-white powder. Solubility: Soluble in dimethyl formamide, DMSO or methanol; slightly soluble in water (0.4mg/ml). Long Term Storage: -20°C. Handling: Protect from light. Keep cool and dry.

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