Thermo Scientific™ Accessing the Phosphoproteome Bundle

Description

Includes

High-Select TiO2 Phosphopeptide Enrichment Kit, High-Select Fe-NTA Phosphopeptide Enrichment Kit

Thermo Scientific Accessing the Phosphoproteome Bundle enables deep phosphoproteome profiling by mass spectrometry through easy to use optimized metal ion affinity chromatography and titanium dioxide chromatography kits.

• High-Select Fe-NTA Phosphopeptide Enrichment Kit enriches phosphopeptides from complex samples using iron-chelate resin in spin columns. Each column can enrich phosphopeptides from 0.5 to 5 mg of total protein digest. Together with pre-formulated buffers, the improved and simplified procedure enriches up to 150µg of phosphopeptides with greater than 90% selectivity in less than 45 minutes
• High-Select TiO2 enrichment kit includes 24 titanium dioxide (TiO2) spin tips with optimized buffers to facilitate preparation of enriched phosphopeptides. Each column can enrich phosphopeptides from 0.5 to 3mg of total protein digest as starting sample. The optimized buffers and simplified procedure eliminate the need for pyrrolidine in the elution buffer and subsequent clean-up using graphite spin columns. Use of this kit results in enrichment of up to 150 µg of phosphopeptides with greater than 90% selectivity
• The High-Select TiO2 Phosphopeptide Enrichment Kit is complementary to the High-Select Fe-NTA Phosphopeptide Enrichment Kit, with both kits enriching similar numbers of phosphopeptides but different subpopulations of phosphorylated peptides

Specifications

• Kit Contents: High-Select TiO2 Phosphopeptide Enrichment Kit, High-Select Fe-NTA Phosphopeptide Enrichment Kit
• Content And Storage: See instructions for individual components
• Quantity: 1 Bundle