Invitrogen™ AccuCheck™ ERF Reference Particles

Catalog No.	Quantity
A55950	3 mL

Catalog No. A55950 Supplier Invitrogen™ Supplier No. A55950

Description

Used for standardization and calibration of an intensity unit of fluorescence for flow cytometry analysis

• AccuCheck ERF Reference Particles are used for standardization and calibration of an intensity unit of fluorescence for flow cytometry analysis.
• Through the application of the assigned NIST-traceable intensity standard for a given fluorophore, instrument sensitivity and accuracy can be determined, permitting data comparison between flow cytometry instruments.
• In combination with a biological standard, these particles can also be used to quantify the level of biomarker expression.
• Includes lot-specific NIST-traceable ERF values assigned to 26 channels
• Enables fluorescence intensity measurements for instrument accuracy and sensitivity
• Facilitates instrument to instrument data comparison

Specifications

• Concentration: 2 x 10^6 beads/ mL
• Content And Storage: Store at 2–8°C
• For Use With (Application): Flow Cytometry
• Material: Polystyrene
• Product Type: ERF Reference Particle
• Flow Cytometer Laser Lines: 405 nm, 488 nm, 561 nm, 640 nm
• Quantity: 3 mL
• Volume (Metric): 3 mL
• Diameter (Metric): 3.1 μm

Frequently Asked Questions (FAQs)

Can AccuCheck ERF Reference Particles (Cat. No. A55950) be used with fluorescence-activated cell sorters?

Yes, AccuCheck ERF Reference Particles (Cat. No. A55950) can be used with fluorescence-activated cell sorters as long as the cell sorter has the appropriate channel/filter(s).

What are the 26 emission filters referenced by the AccuCheck ERF Reference Particles (Cat. No. A55950)?

The emission filters referenced by the AccuCheck ERF Reference Particles (Cat. No. A55950) can be found in Table 1 of the Poster: Internally stained multiple fluorescent microsphere intensity reference with NIST-assigned ERF values for quantitative flow cytometry analysis

I accidentally stored the AccuCheck ERF Reference Particles (Cat. No. A55950) in the freezer, can they still be used?

No. If frozen, the AccuCheck ERF Reference Particles (Cat. No. A55950) may have aggregated to such an extent that the aggregates cannot be adequately dispersed by sonication.

for resarch use only