This kit detects ACACA (Acetyl-CoA carboxylase 1) in human serum plasma tissue homogenates and cell lysates using a sandwich ELISA methodology It offers a quantitative detection range of 9 38 pg/mL to 600 pg/mL with a sensitivity of 2 34 pg/mL ensuring precise measurement of low-abundance samples The assay requires a short incubation period of 1-5 hours and utilizes a sample volume of 50-100 L per test Results are determined by absorbance measurement at 450 nm providing a reliable and standardized approach for ACACA analysis in research or diagnostic applications The kit s optimized protocol maintains specificity and reproducibility across diverse biological matrices making it suitable for studies involving lipid metabolism regulation enzyme activity monitoring or biomarker discovery related to ACACA expression