Thermo Scientific™ ActivX™ TAMRA-FP Serine Hydrolase Probe

Description

ActivX™ probes have been designed to selectively label and enrich serine hydrolases through binding of their respective active sites with tagged fluorophosphonate to enable assessment of enzyme activity.

The TAMRA-FP probe feature a reactive fluorophosphonate group that is used to label and detect active serine hydrolases by fluorescent gel imaging, capillary electrophoresis or mass spectrometry. An anti-TAMRA antibody is also available for immuno-enrichment of TAMRA-FP probe-labeled proteins.

ActivX™ active-site enrichment probes are advantageous for determining active enzyme levels through the use of analog-tagged probes to allow profiling of both inactive and active enzymes. The ActivX probes selectively enrich only those enzymes that are functionally active and biologically relevant at the time of labeling. With selective enrichment, one can identify and profile target enzyme classes across samples or can assess the specificity and affinity of enzyme inhibitors.

The serine hydrolase superfamily is one of the largest, most diverse enzyme families in eukaryotic proteomes. Serine hydrolases are generally grouped into two large families: serine proteases (e.g., trypsin, elastase and thrombin) and metabolic serine hydrolases. Metabolic serine hydrolases are divided into multiple enzyme subclasses (e.g., esterases, lipases, amidases and peptidases) based on structure, catalytic mechanism and substrate preference. These probes can be used to assess activity or screen small molecule inhibitors against enzymes derived from cell lysates, subcellular fractions, tissues and recombinant proteins.

These products are subject to a limited use label license.

Specifications

• Description: ActivX™ TAMRA-FP Serine Hydrolase Probe
• Formulation: TAMRA-fluorophosphonate, MW 680
• Content And Storage: Store at -80°C away from light. Stock solutions in DMSO can be stored at -20°C for 6 months.
• Assay: Protein Quantitation Assay
• Detection Method: Chemiluminescence, Mass Spectrometry, Western Blot
• Substrate Type: Serine Hydrolase Substrate
• Product Line: ActivX™
• Quantity: 6.8 μg
• For Use With (Application): Pull-Down
• Target: Serine Hydrolase
• Type: Fluorophosphonate (FP) probe
• Label or Dye: Other Label(s) or Dye(s)
• Format: Tube, Spin Column

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.