Lonza Walkersville AD1 4D-Nucleofector Y Kit

Supplier:  Lonza Walkersville V4YP1A24

Electroporation-based methods have so far required cells to be in suspension for transfection. However, the 4D-Nucleofector Y Unit allows direct Nucleofection of cells in adherence. Cells which typically grow in adherence in cell culture, can be kept and transfected by Nucleofection in their physiological state. Benefits:Nucleofection of neuronal cells at any time point during their culture period, i.e. at a later developmental stagesNucleofection of cells in adherence without the need for transfer in suspensionTransfection efficiencies up to 70% combined with high viabilitiesApplications:Electroporation of cells in adherenceFor transfection of 2.5 x 10⁴ - 1 x 10⁵ cells Content:2 x 4.5 mL AD1 4D-Nucleofector Y Solution2 x 1 mL Supplement 13 x 150 μg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)1 x 24-well Dipping Electrode Array1 x Nunclon Δ Surface 24-well plate (Nunc)

Catalog No. 50-113-1106