Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
Learn More
VAZYME INTERNATIONAL LLC Add Read cAMP Detection Kit 1 Kit
Supplier: VAZYME INTERNATIONAL LLC DD280101
cAMP Detection Kit is a competitive immunoassay intended to measure cyclic AMP (cAMP) accumulation in cells. The kit contains an antibody that specifically recognizes cAMP, labeled with a fluorescent donor Eu (Anti-cAMP-Eu), and cAMP is labeled with a fluorescent acceptor A2 (cAMP-A2). In the solution, Anti-cAMP-Eu binds with cAMP-A2, which can lead to Fluorescence Resonance Energy Transfer (FRET). When excited with light at 320 nm, the fluorescent donor Eu emits light at 620 nm. This 620 nm light, in turn, excites the fluorescent acceptor A2, causing it to emit light at 665 nm. After cell lysis, the cAMP produced within the cells is released into the lysate sample, which competes for binding with Anti-cAMP-Eu, disrupting the FRET phenomenon.The FRET signal value (the ratio of light intensity at 665 nm to 620 nm) is inversely proportional to the concentration of cAMP in the standard or sample.
Product Title
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.
Spot an opportunity for improvement?