Edvotek™ AIDS Kit I: Simulation of HIV-1 Detection

Description

Includes

Complete instructions, background information, and study questions; biologicals and reagents; three microtiter plates; 50 transfer pipettes; 60 microtest tubes with attached caps; twenty-five 1mL pipettes; two 50mL plastic tubes.

Requires

Requirements:

• Distilled or deionized water
• Beakers z 37°C incubation oven
• Automatic micro- pipettes and tips (optional)

In this experiment, students will perform an ELISA test by coating microtiter plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.

A model for HIV-1 detection without the use of blood

Simulates the screening for antibodies against human immunodeficiency virus (HIV-1). Enzyme-linked immunoabsorbent assay (ELISA) is currently used for initial clinical screening of the blood supply for HIV-1. Students add simulated HIV antigen to wells in the microtiter plate and follow it with washing and introduction of simulated antibodies of HIV-1 positive or negative samples. A second antibody is added which binds to the first antibody of HIV-1 positive samples and results in a color change.

Note: some kit contents may need to be redeemed by mail.

Order Info

Kit ships with plasticware and instructions on how to obtain remaining materials directly from the supplier, Edvotek.

Specifications

• Product Type: AIDS Kit I
• Grade: 9 to Undergraduate
• For Use With (Application): Detects hiv infection indirectly using an elisa test against hiv antibodies in the blood
• Includes: Instructions, simulated hiv antigens, serum samples, antibodies, buffers, microtiter plates, assorted pipets and microtest tubes
• Time Range: 60 min.
• Content And Storage: Refrigerated
• Class Size: 10 Lab Groups