Invitrogen™ Multispecies AKT/ERK Activation InstantOne™ ELISA Kit

Catalog No.	Quantity
50-246-233	96 Tests

Catalog No. 50-246-233 Supplier Invitrogen™ Supplier No. 858601411

Description

ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated human, mouse, and hamster ERK 1/2 and AKT 1/2/3 in cell lysates. Detection of rat ERK 1/2 and AKT 1/2/3 is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

Extracellular signal-regulated kinase (ERK) is the founding member and a key component of the classical Mitogen-Activated Protein Kinase (MAPK) pathway. ERK1 (p44, MAPK3) and ERK2 (p42, MAPK2) are both activated via the MAPK/ERK pathway, which is downstream of various Receptor Tyrosine Kinases (RTKs) or GPCRs. MEK, a Ser/Thr and Tyr kinase, activates ERK by phosphorylation of its TxY motifs, namely Thr202/Tyr204 and Thr185/Tyr187 of ERK1 and ERK2, respectively. Once activated, ERK phosphorylates a variety of proteins that regulate cellular processes such as cell division, proliferation, survival, differentiation, apoptosis, motility, and metabolism. AKT, which exists as multiple isoforms, is one of the principle kinases activated by phosphoinositide 3-kinase (PI3K). Activation of PI3K results in the generation of phosphatidylinositol (3,4,5)-triphosphate (PIP3). These lipid second messengers bind to the pleckstrin homology domain of AKT to promote its translocation to the plasma membrane for activation via phosphorylation at Thr308 and Ser473 by PDK1 and the mTOR TORC2 complex, respectively. Phosphorylation at both these sites is required for full activation of AKT Ser/Thr kinase activity. AKT phosphorylates over 50 known substrates, including GSK3, AS160, PRAS40, TSC 1, TCS 2, Raf-1, Bad, the FOXO family of transcription factors, and PFK2. Due to its established critical role in numerous biological processes, AKT is a frequent target for oncology-related drug development.

Specifications

• Accession Number: P27361, P28482, P31749, P31751, Q9Y243
• Content And Storage: • User Manual
  • phospho-AKT 1/2/3 (Ser437) Capture Antibody Reagent, 1 x 1.5 mL
  • phospho-AKT 1/2/3 (Ser437) Detection Antibody Reagent, 1 x 1.5 mL
  • phospho-ERK 1/2 (Thr202/Tyr204, Thr185/Tyr187) Capture Antibody Reagent, 1 x 1.5 mL
  • phospho-ERK 1/2 (Thr202/Tyr204, Thr185/Tyr187) Detection Antibody Reagent, 1 x 1.5 mL
  • InstantOne ELISA Assay Plate, 96 well (sealed)
  • Cell Lysis Buffer (5X), 10 mL
  • Enhancer Solution, 1 mL
  • Wash Buffer (10X), 15 mL
  • Detection Reagent (TMB Substrate), 12 mL
  • Stop Solution, 12 mL
  • Positive Control Cell Lysate (lyophilized), 0.25 mL
  • Plate seal, 1 ea
• Conjugate: HRP
• Detection Method: Colorimetric
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: ERK; pan AKTERK; pan AKT; AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA;ERK; ERK-2; ERK2; ERT1; MAPK2; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; p42-MAPK;ERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; p44-ERK1; p44-MAPK
• Gene ID (Entrez): 10000, 207, 208, 5594, 5595
• Gene Symbol: AKT1, AKT2, AKT3, MAPK1, MAPK3
• Kit Contents: Capture Antibody, Detection Antibody Reagent, InstantOneTM Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 20 μL
• Storage Requirements: 2-8°C
• Synonym: PKB, RAC-Pk.-alpha / p44/p42, MAPK3/MAPK2
• Target: AKT Pan/Erk1/2
• Test Time: 1 hr 20 min
• Total Assay Time: 1 hr 10 min

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov