Invitrogen™ Multispecies AKT/ERK/p70 S6K Activation InstantOne™ ELISA Kit

Catalog No.	Quantity
50-246-235	96 Tests

Catalog No. 50-246-235 Supplier Invitrogen™ Supplier No. 858601811

Description

ELISA

The InstantOne™ ELISA is specifically engineered for accurate measurement of phosphorylated ERK 1/2 (human, mouse, hamster), AKT 1/2/3 (human, mouse, hamster), and p70 S6K (human, mouse) in cell lysates. Detection of these phosphorylated molecules in rat is also expected. The InstantOne™ ELISA kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich ELISA are added in solution to a plate followed by a wash step and detection with the TMB colorimetric substrate.

Extracellular signal-regulated kinase (ERK) is the founding member and a key component of the classical Mitogen-Activated Protein Kinase (MAPK) pathway. MEK activates ERK 1/2 by phosphorylation of its TxY motifs in response to a variety of stimuli, including growth factors and cytokines. AKT, which exists as multiple isoforms, is one of the principle kinases activated by phosphoinositide 3-kinase (PI3K). These lipid second messengers bind to the pleckstrin homology domain of AKT to promote its translocation to the plasma membrane for activation via phosphorylation at Thr308 and Ser473 by PDK1 and the mTOR TORC2 complex, respectively. Phosphorylation at both these sites is required for full activation of AKT Ser/Thr kinase activity. p70 S6 Kinase (p70S6K) is a member of the ribosomal S6 kinase family of Ser/Thr kinases. p70 S6K activity is controlled by multiple signaling pathways, including the MAPK, phosphoinositide-3 kinase (PI3K), and mTOR pathways. This regulation results in the phosphorylation of multiple sites of p70 S6K, including Thr389 within the linker domain, which is required for full activation. Activated p70 S6K phosphorylates several residues on the S6 ribosomal protein, thereby leading to increased protein synthesis.

Specifications

• Accession Number: P23443, P27361, P28482, P31749, P31751, Q9Y243
• Content And Storage: • User Manual
  • phospho-ERK 1/2 (Thr202/Tyr204, Thr185/Tyr187) Capture Antibody Reagent, 1 mL
  • phospho-ERK 1/2 (Thr202/Tyr204, Thr185/Tyr187) Detection Antibody Reagent, 1 mL
  • phospho-AKT 1/2/3 (Ser437) Capture Antibody Reagent, 1 mL
  • phospho-AKT 1/2/3 (Ser437) Detection Antibody Reagent, 1 mL
  • phospho-p70 S6K (Thr389) Capture Antibody Reagent, 1 mL
  • phospho-p70 S6K (Thr389) Detection Antibody Reagent, 1 mL
  • InstantOne Assay Plate, 96 well (sealed)
  • Cell Lysis Buffer (5X), 10 mL
  • Enhancer Solution, 1 mL
  • Wash Buffer (10X), 15 mL
  • Detection Reagent (TMB Substrate), 12 mL
  • Stop Solution, 12 mL
  • Positive Control Cell Lysate (lyophilized), 0.25 mL
  • Plate seal, 1 ea
• Conjugate: HRP
• Detection Method: Colorimetric
• Immunoassay Kit Format: Coated ELISA Kit
• Product Type: ELISA
• Sample Type: Cell Lysates
• Target Species: Human
• For Use With (Application): ELISA
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: ERK; pan AKT; s6kERK; pan AKT; s6k; AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA;ERK; ERK-2; ERK2; ERT1; MAPK2; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; p42-MAPK;PS6K; S6K; S6K-beta-1; S6K1; STK14A; p70 S6KA; p70(S6K)-alpha; p70-S6K; p70-alpha;ERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A
• Gene ID (Entrez): 10000, 207, 208, 5594, 5595, 6198
• Gene Symbol: AKT1, AKT2, AKT3, MAPK1, MAPK3, RPS6KB1
• Kit Contents: Capture Antibody, Detection Antibody Reagent, InstantOneTM Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal, Detection Antibody Reagent, InstantOne™ Assay Plate, Cell Lysis Buffer, Enhancer Solution, Wash Buffer (10X), Substrate Reagent (TMB), Stop Solution, Positive Control Cell Lysate (lyophilized), Plate seal
• Label or Dye: Biotin
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 20 μL
• Storage Requirements: 2-8°C
• Target: AKT Pan/Erk1/2/p70 S6 Kinase
• Test Time: 1 hr 20 min
• Total Assay Time: 1 hr 10 min