Invitrogen™ Human AKT (Phospho) [pS473] ELISA Kit

Catalog No.	Quantity
KHO0111	96 Tests

Catalog No. KHO0111 Supplier Invitrogen™ Supplier No. KHO0111

Description

ELISA

The Human AKT (Phospho) [pS473] ELISA Kit is a solid-phase sandwich Enzyme-Linked Immunosorbent Assay (ELISA) designed to detect and quantify the level of AKT (Phospho) [pS473] in fresh or frozen human cell lysates. The assay recognizes both natural and recombinant AKT (Phospho) [pS473]. Principle of the method A monoclonal capture antibody specific for AKT (Phospho) [pS473] has been coated onto the wells of the 96-well plate. During the first incubation, standards of known content and unknown samples are pipetted into the wells and the antigen binds to the immobilized (capture) antibody. After washing, a rabbit antibody specific for the target protein is added to the wells and serves as a detection antibody by binding to the immobilized protein captured during the first incubation. After washing, a horseradish peroxidase labeled anti-rabbit IgG is added. This binds to the detection antibody to complete the four member sandwich. After a third incubation and washing to remove all the unbound enzyme, a substrate solution (TMB) is added, which is acted upon by the bound enzyme to produce color. The intensity of this colored product is directly proportional to the concentration of target protein present in the original specimen and the optical density can be read on a standard microplate reader. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to...

AKT1 (PKB alpha) is a serine/threonine kinase that regulates cell survival. The activated enzyme inhibits apoptosis and stimulates cell cycle progression by phosphorylating numerous targets in various cell types, including cancer cells. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

Specifications

• Accession Number: P31749
• Assay Range: 1.6-100 U/mL
• Assay Sensitivity: <0.8 U/mL
• Concentration: 1.6 U/mL
• Content And Storage: Pre-coated plate(s), standard or calibrator, detector antibody, HRP conjugate, diluents, wash buffer, substrate(s), and stop solution. Store kit at 2-8°C. See product manual for detailed contents and storage conditions for maximum stability.
• Conjugate: HRP
• Detection Method: Colorimetric
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: Kinases & Inhibitors
• Sample Type: Cell Lysates
• Substrate Type: Serine/Threonine Kinases
• Target Species: Human, Mouse, Human
• For Use With (Application): Cell Analysis, Cell Signaling, Cell Viability, Proliferation and Function, Drug Discovery and Development, Immunoassays, Kinase Antibodies and Immunoassays, Kinase Biology, Ready-To-Use Immunoassay, Target and Lead Identification and Validation
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: Akt; Akt kinase; AKT serine/threonine kinase 1; Akt/PKB; akt1; AKT1 kinase; AKT1m; Akt1m protein; Akt1-PA; Akt1-PB; Akt1-PC; Akt1-PD; Akt1-PE; CAKT; C-AKT; CG4006; CG4006-PA; CG4006-PB; CG4006-PC; CG4006-PD; CG4006-PE; CWS6; dAkt; D-Akt; dAkt kinase; dAKT/dPKB; dAkt/PKB; DAKT1; DAKT1/PKB; Dmel\CG4006; Dmel_CG4006; dPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq; murine thymoma viral (v-akt) oncogene homolog 1; pAkt; P-AKT; PKB; PKB alpha; PKB beta; PKB gamma; PKB/AKT; PKB/dAKT; PKBalpha; PKB-ALPHA; PKBG; PRKBA; protein kinase B; Protein kinase B alpha; protein kinase B-alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC serine/threonine protein kinase; RAC serine/threonine-protein kinase; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RacPK; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; related to PKA to PKC protein kinases; related to the A and C kinases; SD10374p; serine/threonine protein kinase; serine-threonine protein kinase; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1Akt; Akt kinase; AKT serine/threonine kinase 1; Akt/PKB; akt1; AKT1 kinase; AKT1m; Akt1m protein; Akt1-PA; Akt1-PB; Akt1-PC; Akt1-PD; Akt1-PE; CAKT; C-AKT; CG4006; CG4006-PA; CG4006-PB; CG4006-PC; CG4006-PD; CG4006-PE; CWS6; dAkt; D-Akt; dAkt kinase; dAKT/dPKB; dAkt/PKB; DAKT1; DAKT1/PKB; Dmel\CG4006; Dmel_CG4006; dPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq; murine thymoma viral (v-akt) oncogene homolog 1; pAkt; P-AKT; PKB; PKB alpha; PKB beta; PKB gamma; PKB/AKT; PKB/dAKT; PKBalpha; PKB-ALPHA; PKBG; PRKBA; protein kinase B; Protein kinase B alpha; protein kinase B-alpha; proto-oncogene c-Akt; RAC; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; RAC serine/threonine protein kinase; RAC serine/threonine-protein kinase; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RacPK; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; related to PKA to PKC protein kinases; related to the A and C kinases; SD10374p; serine/threonine protein kinase; serine-threonine protein kinase; Thymoma viral proto-oncogene; thymoma viral proto-oncogene 1; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1; AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA;HIHGHH; PKBB; PKBBETA; PRKBB; RAC-BETA;MPPH; MPPH2; PKB-GAMMA; PKBG; PRKBG; RAC-PK-gamma; RAC-gamma; STK-2
• Gene ID (Entrez): 207
• Gene Symbol: AKT1
• Incubation Time: 4 hr.
• Interassay CV: 8.3%
• Intraassay CV: 6.9%
• Kit Contents: Pre-coated 96 well plate, Standard, Standard Dilution Buffer, Detection Antibody, anti-rabbit-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Standard Dilution Buffer, Detection Antibody, anti-rabbit-HRP, HRP Diluent, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: HRP
• Marker Gene: Akt
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Cell Lysate 10 μL
• Shipping Condition: Wet Ice
• Storage Requirements: 2-8°C
• Target: AKT1
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov