Enzo Life Sciences Akt kinase activity kit (1x96wells)
Manufacturer: Enzo Life Sciences ADIEKS400A
This is a non-radioactive Akt kinase activity assay, providing a safe, rapid and reliable method for the screening of inhibitors or activators of Akt and for quantitating the activity of Akt in purified or partially purified enzyme preparations. The Akt Kinase activity kit is based on a solid phase enzyme immunoassay (EIA) that utilizes a specific synthetic peptide as a substrate for Akt and a polyclonal antibody that recognizes the phosphorylated form of the substrate. The assay is designed for the analysis of Akt (1, 2, 3) activity in the solution phase. Alternative Name: PKB, Protein kinase B. Assay Time: 4.5 hours. Applications: Colorimetric detection, Activity assay. Species Reactivity: Species independent. Contents: Microtiter plate, Antibody, Conjugate, Antibody dilution buffer, Kinase assay dilution buffer, ATP, Active Kinase, Wash buffer concentrate, TMB Substrate, Stop solution 2
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