Human Akt1 (RAC-alpha serine/threonine-protein kinase) is a member of the AGC Ser/Thr protein kinase family that plays a critical role in regulating cellular metabolism, proliferation, cell survival, and angiogenesis. This recombinant protein is expressed in HEK293 cells and contains a C-terminal polyhistidine tag followed by a twin strep tag. It is supplied as a lyophilized powder formulated in 50 mM Tris, 150 mM NaCl, pH 8.0, with trehalose as a protectant.
- Purity >90% as determined by SDS-PAGE
- Calculated molecular weight of 59.5 kDa, migrating as 60–66 kDa under reducing conditions due to glycosylation
- Endotoxin level less than 1.0 EU per μg by the LAL or rFC method
- Expressed from human HEK293 cells covering amino acids Met 1 to Ala 480
- Stable for 12 months in lyophilized state when stored at -20°C or lower