Invitrogen™ Human ALCAM/CD166 ELISA Kit

Catalog No.	Quantity
EHALCAMX5	5 x 96 Tests
EHALCAM	96 Tests

Catalog No. EHALCAMX5 Supplier Invitrogen™ Supplier No. EHALCAMX5

Description

ELISA

The Human ALCAM/CD166 (Hu ALCAM) ELISA quantitates Hu ALCAM in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu ALCAM. Principle of the method The Human ALCAM solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene encodes activated leukocyte cell adhesion molecule (ALCAM), also known as CD166 (cluster of differentiation 166), which is a member of a subfamily of immunoglobulin receptors with five immunoglobulin-like domains (VVC2C2C2) in the extracellular domain. This protein binds to T-cell differentiation antigene CD6, and is implicated in the processes of cell adhesion and migration. Multiple alternatively spliced transcript variants encoding different isoforms have been found.

Specifications

• Accession Number: Q13740
• Assay Range: 15-5000 pg/mL
• Assay Sensitivity: 15 pg/mL
• Calibration Range: 15 pg/mL to 5000 pg/mL
• Assay: Solid-phase sandwich ELISA
• Conjugate: HRP
• Immunoassay Kit Format: Sandwich ELISA Kit
• Product Type: ELISA
• Protein Family: CD Proteins
• Sample Type: Plasma, Serum, Supernatant
• Specificity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL- 11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP- 1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
• Sufficient For: 5 x 96 assays
• Target Species: Human
• For Use With (Application): ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis
• For Use With (Equipment): Absorbance Microplate Reader
• Gene Alias: activated leukocyte cell adhesion molecule; AI853494; ALCAM; BEN; CD166; CD166 antigen; cell adhesion molecule; DM-GRASP; FLJ38514; HB2; KG-CAM; MEMD; MGC71733; MuSC; Protein DM-GRASP; Protein MEMD; SB-10 antigen; SC1; unnamed protein productactivated leukocyte cell adhesion molecule; AI853494; ALCAM; BEN; CD166; CD166 antigen; cell adhesion molecule; DM-GRASP; FLJ38514; HB2; KG-CAM; MEMD; MGC71733; MuSC; Protein DM-GRASP; Protein MEMD; SB-10 antigen; SC1; unnamed protein product; CD166; MEMD
• Gene ID (Entrez): 214
• Gene Symbol: ALCAM
• Interassay CV: <12%
• Intraassay CV: <10%
• Kit Contents: Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers
• Label or Dye: Biotin
• Protein Aliases: Activated leukocyte cell adhesion molecule
• Quantity: 5 x 96 Tests
• Regulatory Status: RUO
• Research Discipline: Extracellular Matrix & Cell Adhesion
• Sample Volume: Plasma 2 μL, Serum 2 μL, Supernatant 100 μL
• Shipping Condition: Wet or Dry Ice
• Storage Requirements: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
• Synonym: CD166, MEMD
• Target: CD166 (ALCAM)
• Test Time: 1 hr 20 min
• Total Assay Time: 4 hr 45 min