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Alcian Blue Solution, Sigma-Aldrich

Catalog No. 01259010 Shop All MilliporeSigma Supelco Products
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01-259-010 250 mL
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Catalog No. 01-259-010 Supplier MilliporeSigma Supelco Supplier No. B8438250ML
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Description

Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2. 5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.

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Specifications

Chemical Name or Material Alcian Blue solution
Concentration 1%
Percent Purity ≥85 %
Color Dark Blue
pH 2.4 to 2.6
Quantity 250 mL
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