Invitrogen™ Alexa Fluor™ 488 Tyramide SuperBoost™ Kit, goat anti-rabbit IgG

Description

Features poly-HRP-conjugated goat anti-rabbit IgG secondary antibody. Alexa Fluor™ 488 Tyramide SuperBoost™ Kit combine brightness of AlexaFluor dyes with high signal amplification of a poly-HRP-mediated tyramide labeling reaction to produce s

SuperBoost™ tyramide signal amplification is most sensitive method for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

Features:

• High sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images, co-label with DAPI, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments

Benefits:

• Enhancement of signal using Alexa Fluor tyramides: Utilize Alexa Fluor tyramides, which react with HRP to ultimately deposit bright and photostable Alexa Fluor dye on surrounding proteins and other similar molecules
• Poly-HRP enhancement: Several HRP enzymes are conjugated with short polymers, enhancing signal by several fold over regular HRP systems
• Reaction stop solution: Include an HRP stop solution to halt the HRP reaction
• Reduction of background: Include blockers for elimination or reduction of endogenous peroxidase and fluorescent background signals

Shelf Life:

• Stable for 6 months after receiving

Specifications

• Shipping Condition: Approved for shipment at room temperature or on wet ice
• Content And Storage: 1 kit sufficient for 50 microscope slides (18 x 18 mm), containing:
  
  • Blocking buffer (1X), 22.5 mL
  • Poly-HRP-conjugated goat anti-rabbit secondary antibody (1X), 22.5 mL
  • Alexa Fluor tyramide reagent
  • Hydrogen peroxide (stabilized 3% solution), 28.5 mL
  • Reaction buffer (20X), 6 mL
  • Reaction stop solution, 8 mg
  • Dimethylsulfoxide (DMSO), 200 μL
  
  Store at 2-8°C. When stored as directed, the product is stable for 6 months after receiving.
• Quantity: 50 Slides
• Conjugate: Alexa Fluor 488

For Research Use Only. Not for use in diagnostic procedures.