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Molecular Probes™ Alexa Fluor™ 546 Protein Labeling Kit Non-distribution product as customer accommodation.

Alexa Fluor™ 546 Protein Labeling Kit

Manufacturer:  Molecular Probes™ A10237

Catalog No. A10237


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Description

Description

Molecular Probes™ Protein Labeling Kits provide a convenient means for attaching a fluorescent label (or biotin) to an antibody (or a protein larger than 40 kDa). Conjugates are ideal for multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, FISH, and more. Kits are available in 12 Alexa Fluor™ dye colors, biotin, the hapten Oregon Green™ 488, fluorescein EX, and Texas Red™ dye. Each kit provides the components needed to perform three protein conjugations and purifications.

Important Features of Protein Labeling Kits:

  • Labeled proteins typically ready to use in 2 hr (~30 min hands-on time)
  • Designed to label 1 mg of IgG
  • Simple protocol — react, separate, use
  • Stabilizing proteins must be removed from the sample before labeling


    The Benefits of Alexa Fluor™ Dyes
    Compared to traditional dyes, Alexa Fluor™ dyes are brighter, more photostable, and more pH resistant between pH 4 and 10. And generally when using Alexa Fluor™ dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor™ Dyes Spanning the Visible and Infrared Spectrum—Section 1.3.

    Learn More About Protein and Antibody Labeling
    We offer a wide selection of Molecular Probes™ antibody and protein labeling kits to fit your starting material and your experimental setup. See Antibody Labeling from A to Z or use our Labeling Chemistry Selection Tool for other choices. To learn more about our various kits read Kits for Labeling Proteins and Nucleic Acids—Section 1.2 in the Molecular Probes™ Handbook.

    We’ll Make a Custom Antibody Conjugate for You
    If you can’t find what you’re looking for in our stocked list, we’ll prepare a custom antibody conjugate for you. Our custom conjugation service is efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.

    For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.
  • Specifications

    Specifications

    Alexa Fluor™ 546 Protein Labeling Kit, Orange, Fluorescent Detection Method, Alexa Fluor™ 546 Dye, C50H62Cl3N5O14S3 Molecular Formula, 1159.6 Molecular Weight, 556/573nm Excitation/Emission Wavelength, Used for Labeling Chemistry, Protein Labe
    Labeling chemistry, protein labeling and crosslinking
    Alexa Fluor™ 546 reactive dye (Component A), 3 vials, each containing a magnetic stir bar, sodium bicarbonate (MW = 84, component B), 84mg, purification resin (component C Bio-Rad BioGel™ P-30 fine size exclusion purification resin) ∽25mL
    4 to 10
    2 to 8°C
    1159.6
    573nm
    Orange
    Alexa Fluor™ 546
    Alexa Fluor™ 546 reactive dye (Component A), 3 vials, each containing a magnetic stir bar, sodium bicarbonate (MW = 84, component B), 84mg, purification resin (component C Bio-Rad BioGel™ P-30 fine size exclusion purification resin) ∽25mL
    Alexa Fluor™ 546 Protein Labeling Kit
    1 Kit(s)
    556nm
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    For Research Use Only. Not for use in diagnostic procedures.