Invitrogen™ Alexa Fluor™ 647-Poly-GT Peptide Substrate

Description

A general AlexaFluor647-labeled peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase acitivty.

A general AlexaFluor647-labeled peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase acitivty.

For use in combination with the LanthaScreen™ Eu-PY20 Antibody (Cat. No. PV5696) to evaluate inhibitors in medium to high throughput screening applications.

Specifications

• Assay Entry: Biochemical europium phospho-specific activity
• Color: Far-Red
• Content And Storage: 30 μM AlexaFluor 647 Poly-GT peptide substrate in 10 mM HEPES, 137 mM NaCl, 2.7 mM KCl, pH 7.5 (HEPES buffered saline).
  
  Store at -20°C and protect from light.
• Detection Method: Fluorescence
• Form: Liquid
• Product Line: LanthaScreen™
• Quantity: 1 nmol
• Substrate: Random (Glu:Tyr) polymer in a ratio of 4:1
• Substrate Properties: Peptide-Based Substrate
• Substrate Type: Kinase Substrate
• Target Enzyme: Tyrosine Kinases
• Excitation/Emission: 340/665 nm
• Label or Dye: Alexa Fluor™ 647
• Shipping Condition: Dry Ice
• Target Entry: ABL1 G250E, CSF1R, CSK, EPHA5, KIT, PDGFRA V561D, SYK, TYRO3, ZAP70, FGFR1, FGFR2, EGFR, EPHB2, FGFR4, IGF1R, LYNA, MATK, MERTK, NTRK3, RET V804L, TEK, FLT1, MUSK, SRCN1, ABL1 E255K, EPHA4, FYN, PTK2, PTK2B, RET, ABL1 T315I, EPHA3, EPHB4, FLT3, FLT4, INSR, LCK, YES1, EGFR L858R, ERBB4, KDR, MET, TNK2, ABL1, ABL2, AXL, FGFR3, FGR, HCK, KIT V654A, EPHA1, EPHA8, EPHB3, INSRR, LYNB, NTRK1, PDGFRA D842V, SRMS, EGFR L861Q, EPHA2, FER, LTK, PDGFRA, EPHA7, EPHB1, FRK, ITK, FLT3 D835Y, SRC, BTK, FES, ERBB2, NTRK2
• Technique: TR-FRET
• Validated Kinases: EGFR

For Research Use Only. Not for use in diagnostic procedures.