Abnova™ ALK Split CISH Probe

Catalog No.	For Use With (Application)
89-335-532	CISH-P

Catalog No. 89-335-532 Supplier Abnova™ Supplier No. CS0001

Description

ALK Split CISH Probe is designed for the qualitative detection of human ALK gene at 2p23.2 in formalin-fixed, paraffin-embedded specimens by chromogenic in situ hybridization (CISH).

The probe is intended to be used in combination with the CISH Implementation Kit 2, which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear dark green distinct dot-shaped (proximal to the ALK breakpoint region), and dinitrophenyl-labeled polynucleotides appear bright red distinct dot-shaped (distal to the ALK breakpoint region).
Normal situation: In interphases of normal cells or cells without a translocation involving the ALK gene region, two red/green fusion signals appear.
Aberrant situation: One ALK gene region affected by a translocation is indicated by one separate green signal and one separate red signal. EML4-ALK inversion with deletion of 5'-ALK sequences is indicated by one or multiple isolated red signals.
Other signal distribution may be observed in some abnormal samples which might result in a different signal pattern than described above, indicating variant rearrangements.
Unexpected signal patterns should be further investigated.

Specifications

• Content And Storage: The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18 to 25°C) and mix briefly before use.
• For Use With (Application): CISH-P