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Apexbio Technology LLC HyperScribe™ All in One mRNA Synthesis Kit Plus 1 (ARCA, 5mCTP, UTP, T7, poly(A)), 25rxns
Supplier: Apexbio Technology LLC K106425
HyperScribe™ All in One mRNA Synthesis Kit Plus 1 (ARCA, 5mCTP, UTP, T7, poly(A)) is designed for quick production of ARCA capped, 5mCTP and UTP modified, poly(A) tailed mRNA in vitro. Capped mRNA is synthesized by co-transcriptional incorporation of Anti-Reverse Cap Analog (ARCA, Catalog No. B8175) using T7 RNA Polymerase. The capping of ARCA ensures high translation efficacy. The addition of 5mCTP (Catalog No. B7967) / UTP (Catalog No. B7972) can reduce host cell immune response. After a brief DNase I treatment to remove the template DNA, capped and modified mRNA is poly(A) tailed with Poly(A) Polymerase. Polyadenylation (i.e. addition of Poly(A) tail) plays an important role in the stabilization of RNA in eukaryotes and enhances the efficiency of translation initiation. The kit contains sufficient reagents to carry out 25 reactions, 20 l each time. Up to 25-180 g of RNA can be generated with 1 g of control template per standard reaction.
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