Invitrogen™ Allopregnanolone ELISA Kit

Description

ELISA

The Allopregnanolone Competitive ELISA quantitates allopregnanolone in serum, plasma, urine, saliva, dried fecal extracts or cell culture medium.∣Principle of the method: The Progesterone Metabolites Competitive ELISA research-use-only kit is designed to quantitatively measure allopregnanolone independent of species. A allopregnanolone standard is provided to generate a standard curve for the assay and all samples are read off a user-generated standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with anti-rabbit IgG antibodies. Anti-rabbit allopregnanolone antibodies and allopregnanolone-peroxidase conjugate is added to the wells. The allopregnanolone-peroxidase conjugate and any allopregnanolone in the sample will compete to bind to the anti-rabbit allopregnanolone antibodies. After incubation, the plate is washed and substrate is added. The substrate reacts with the bound allopregnanolone-peroxidase conjugate. After a 30 min incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader at 450 nm. The intensity of the generated color corresponds inversely to the amount of allopregnanolone present in the sample.∣Rigorous validation: Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Specifications

• Accession Number: 3 alpha,5 alpha-tetrahydroprogesterone; 3 alpha-hydroxy-5 alpha-pregnan-20-one; allopregnanolone; Brexanolone; C21H34O2; Zulresso
• Assay Range: 156.25 to 10,000 pg/mL
• Assay Sensitivity: 129.7 pg/mL
• Conjugate: HRP
• Product Type: ELISA
• Sample Type: Fecal Extract, Plasma, Serum, Supernatant, Urine
• For Use With (Equipment): Colorimetric Microplate Reader
• Interassay CV: 8%
• Intraassay CV: 7
• Kit Contents: Anti-IgG coated 96 well plate, Standard, Assay Buffer Concentrate, Capture Antibody, HRP-conjugated Antigen, Wash Buffer Concentrate, TMB Substrate, Stop Solution, Plate Sealer
• Label or Dye: HRP
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Fecal Extract 50 μL, Plasma 50 μL, Serum 50 μL, Supernatant 50 μL, Urine 12.5 μL
• Storage Requirements: -20°C
• Target: Allopregnanolone
• Test Time: 45 mins
• Total Assay Time: 16 hrs 45 mins