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ELABSCIENCE Alanine Aminotransferase (ALT/GPT) Activity Assay Kit, 100 Assays
Supplier: ELABSCIENCE EBCK235S100T
ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37°C. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.
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