AluI is a restriction enzyme that recognizes AG^CT sites. 10X Cutting Buffer is included that contains BSA, which enhances enzyme stability and binds to contaminants in DNA preps. AluI is a restriction enzyme that recognizes AG^CT sites. 10X Cutting Buffer is included that contains BSA, which enhances enzyme stability and binds to contaminants in DNA preps. Concentration: AluI 10,000 units/ml Includes 10X Cutting Buffer. Formulation: Liquid. In 100mM KCl, containing 10mM Tris-HCl, pH 7.4, 1mM DTT, 0.1mM EDTA, 50% glycerol and 200µg/ml BSA. After reconstitution, 1X Cutting Buffer: 50mM potassium acetate, 20mM Tris-acetate, 10mM magnesium acetate, 100µg/ml BSA, pH 7.9, at 25°C. Source: Produced in an E. coli strain that carries the cloned AluI gene from Arthrobacter luteus. Long Term Storage: -20°C. Applications: CGH, Genotyping, Cloning, Southern Blot.